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- 抗原
- Abeta-pE3
- 适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- Specific for Abeta-pE3.
- 纯化方法
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- 免疫原
- Synthetic peptide pyr-EFRHD coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
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- 应用备注
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WB: 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500
IHC-P: 1 : 200 - 说明
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WB: Detects purified Abeta pE3. Complex samples like brain extracts still have to be tested.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- 缓冲液
- PBS
- 注意事项
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- 储存条件
- -20 °C
- 储存方法
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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Glutaminyl cyclase in human cortex: correlation with (pGlu)-amyloid-? load and cognitive decline in Alzheimer's disease." in: Journal of Alzheimer's disease : JAD, Vol. 39, Issue 2, pp. 385-400, (2014) (PubMed).
: "Glutaminyl cyclase-mediated toxicity of pyroglutamate-beta amyloid induces striatal neurodegeneration." in: BMC neuroscience, Vol. 14, pp. 108, (2013) (PubMed).
: "Prion-like behaviour and tau-dependent cytotoxicity of pyroglutamylated amyloid-β." in: Nature, Vol. 485, Issue 7400, pp. 651-5, (2012) (PubMed).
: "Involvement of perineuronal and perisynaptic extracellular matrix in Alzheimer's disease neuropathology." in: Brain pathology (Zurich, Switzerland), Vol. 22, Issue 4, pp. 547-61, (2012) (PubMed).
: "Glutaminyl cyclase contributes to the formation of focal and diffuse pyroglutamate (pGlu)-A? deposits in hippocampus via distinct cellular mechanisms." in: Acta neuropathologica, Vol. 121, Issue 6, pp. 705-19, (2011) (PubMed).
: "Selective hippocampal neurodegeneration in transgenic mice expressing small amounts of truncated Aβ is induced by pyroglutamate-Aβ formation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 36, pp. 12790-801, (2011) (PubMed).
: "
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Glutaminyl cyclase in human cortex: correlation with (pGlu)-amyloid-? load and cognitive decline in Alzheimer's disease." in: Journal of Alzheimer's disease : JAD, Vol. 39, Issue 2, pp. 385-400, (2014) (PubMed).
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- 抗原
- Abeta-pE3
- 背景
- Synonyms: pyro-Glu Abeta, pE-Abeta, pGlu-Abeta, Abeta(3(pE)-40/42)
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