BRAF 抗体
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北京 101111
Quick Overview for BRAF 抗体 (ABIN1685167)
抗原
See all BRAF 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Purified
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过滤
- Sterile filtered
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免疫原
- Polyclonal BRAF antibody was produced from sera of rabbits immunized with synthetic protein containing BRAF antigenic determinants.
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亚型
- IgG
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应用备注
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ELISA: Use at a concentration range of 1-2 µg/mL.
Western blot: A suitable range of concentrations of this antibody for WB detection is 10 µg/mL.
IHC: A suitable range of concentrations of this antibody for IHC is 10 µg/mL. Immunohistochemical analysis of BRAF expression in paraffin embedded formalin fixed Rat brain tissue. ABIN1685167 was used as primary antibody, dilution 1:200. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
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缓冲液
- PBS solution at a concentration of 1 mg/mL.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Recombinant human CD117, as supplied, can be stored in working aliquots at - 8 °C for one month, or at -20 °C to -70 °C for twelve months.
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有效期
- 12 months
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- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
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别名
- BRAF
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背景
- The c-Kit receptor consists of an extracellular portion with five immunoglobulin-like regions and an intracellular tyrosine kinase domain. The first three immunoglobulin-like domains form a binding site for its ligand, stem cell factor (SCF), the fourth is involved in c-Kit receptor dimerization, and the function of the fifth is, as yet, unknown. Binding of SCF induces homodimerization of the c-Kit receptor, followed by an increase in receptor autophosphorylation activity and recruitment of the c-Kit signaling complex.
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UniProt
- P15056
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途径
- MAPK Pathway, RTK signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
抗原
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