AMPKalpha1 Antibody, pAb, Rabbit detects endogenous levels of human AMPKalpha1 and does not cross-react with AMPKalpha2. It is predicted to react with mouse AMPKalpha1 according to sequence homology. Positive Control: HEK293 and MCF-7
交叉反应 (详细)
AMPKα1 Antibody, pAb, Rabbit detects endogenous levels of human AMPKα1 and does not cross-react with AMPKα2. It is predicted to react with mouse AMPKα1 according to sequence homology.Positive Control: HEK293 and MCF-7
纯化方法
Immunoaffinity chromatography
免疫原
KLH-coupled synthetic peptide within AA 500–550 of human AMPKα1
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Western blot: 0.5-1 µg/mL Other Applications: User optimized
限制
仅限研究用
状态
Lyophilized
缓冲液
PBS, pH 7.4, containing 0.02 % sodium azide
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
储存条件
4 °C/-20 °C
储存方法
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
AMP-activated protein kinase (AMPK) plays an important role in the regulation of energy homeostasis. It is activated by an elevated AMP/ATP ratio caused by cellular and environmental stress, such as heat shock, hypoxia, and ischemia. AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits, each of which is encoded by two or three distinct genes (alpha1, alpha2, beta1, beta2, gamma1, gamma2, gamma3). Phosphorylation of AMPKalpha at Thr172 is the major regulatory site which is required for AMPK activation. AMPKalpha1 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 50050 of human AMPKalpha1 (Swiss Prot: Q13131).