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PRKAA1 抗体 (AA 500-550)

PRKAA1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1574209
发货至: 中国
  • 抗原 See all PRKAA1 抗体
    PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))
    抗原表位
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    AA 500-550
    适用
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    人, 小鼠
    宿主
    • 156
    • 17
    • 1
    克隆类型
    • 143
    • 31
    多克隆
    标记
    • 86
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    This PRKAA1 antibody is un-conjugated
    应用范围
    • 137
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    Western Blotting (WB)
    特异性
    AMPKalpha1 Antibody, pAb, Rabbit detects endogenous levels of human AMPKalpha1 and does not cross-react with AMPKalpha2. It is predicted to react with mouse AMPKalpha1 according to sequence homology. Positive Control: HEK293 and MCF-7
    交叉反应 (详细)
    AMPKα1 Antibody, pAb, Rabbit detects endogenous levels of human AMPKα1 and does not cross-react with AMPKα2. It is predicted to react with mouse AMPKα1 according to sequence homology.Positive Control: HEK293 and MCF-7
    纯化方法
    Immunoaffinity chromatography
    免疫原
    KLH-coupled synthetic peptide within AA 500–550 of human AMPKα1
    亚型
    IgG
    Top Product
    Discover our top product PRKAA1 Primary Antibody
  • 应用备注
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    Western blot: 0.5-1 µg/mL Other Applications: User optimized
    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    PBS, pH 7.4, containing 0.02 % sodium azide
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    储存条件
    4 °C/-20 °C
    储存方法
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • 抗原
    PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))
    别名
    AMPKalpha1 (PRKAA1 产品)
    别名
    AMPK antibody, AMPKa1 antibody, cb116 antibody, im:7154392 antibody, sb:cb130 antibody, wu:fa94c10 antibody, AMPK1 antibody, AMPKalpha1 antibody, AI194361 antibody, AI450832 antibody, AL024255 antibody, C130083N04Rik antibody, ampk antibody, ampka1 antibody, PRKAA1 antibody, protein kinase AMP-activated catalytic subunit alpha 1 antibody, protein kinase, AMP-activated, alpha 1 catalytic subunit antibody, protein kinase, AMP-activated, alpha 1 catalytic subunit L homeolog antibody, PRKAA1 antibody, prkaa1 antibody, Prkaa1 antibody, prkaa1.L antibody
    背景
    AMP-activated protein kinase (AMPK) plays an important role in the regulation of energy homeostasis. It is activated by an elevated AMP/ATP ratio caused by cellular and environmental stress, such as heat shock, hypoxia, and ischemia. AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits, each of which is encoded by two or three distinct genes (alpha1, alpha2, beta1, beta2, gamma1, gamma2, gamma3). Phosphorylation of AMPKalpha at Thr172 is the major regulatory site which is required for AMPK activation. AMPKalpha1 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 50050 of human AMPKalpha1 (Swiss Prot: Q13131).
    途径
    AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect
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