Protein A 抗体 (HRP)
(3 references)-
- 抗原 See all Protein A 抗体
- Protein A
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适用
- Staphylococcus aureus
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宿主
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小鸡
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克隆类型
- 多克隆
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标记
- This Protein A antibody is conjugated to HRP
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应用范围
- ELISA, Western Blotting (WB)
- 特异性
- Chicken Anti-Protein A [HRP] Polyclonal Antibody is specific to protein A.
- 免疫原
- Purified recombinant protein A
- 亚型
- IgG
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anti-Protein A antibody
适用: Staphylococcus aureus IP, Con 宿主: 山羊 Polyclonal unconjugated
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IP, DB 宿主: 山羊 Polyclonal unconjugated
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein A antibody (Texas Red (TR))适用: Staphylococcus aureus FACS, FM 宿主: 山羊 Polyclonal Texas Red (TR)
anti-Protein A antibody (HRP)适用: Staphylococcus aureus ELISA, WB, IHC 宿主: 山羊 Polyclonal HRP
anti-Protein A antibody (FITC)适用: Staphylococcus aureus FACS 宿主: 山羊 Polyclonal FITC
anti-Protein A antibody (Biotin)适用: Staphylococcus aureus ELISA, WB, IHC 宿主: 山羊 Polyclonal Biotin
anti-Protein A antibody (Texas Red (TR))适用: Staphylococcus aureus FACS, IM 宿主: 山羊 Polyclonal Texas Red (TR)
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.05-1.0 µg/mL
Other applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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: "Protein A is released into the Staphylococcus aureus culture supernatant with an unprocessed sorting signal." in: Infection and immunity, Vol. 83, Issue 4, pp. 1598-609, (2015) (PubMed).
: "Detection and quantification of Panton-Valentine leukocidin in Staphylococcus aureus cultures by ELISA and Western blotting: diethylpyrocarbonate inhibits binding of protein A to IgG." in: Journal of immunological methods, Vol. 356, Issue 1-2, pp. 1-5, (2010) (PubMed).
: "Overexpression of liver-specific cytochrome P4502E1 impairs hepatic insulin signaling in a transgenic mouse model of nonalcoholic fatty liver disease." in: European journal of gastroenterology & hepatology, Vol. 21, Issue 9, pp. 973-83, (2009) (PubMed).
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: "Protein A is released into the Staphylococcus aureus culture supernatant with an unprocessed sorting signal." in: Infection and immunity, Vol. 83, Issue 4, pp. 1598-609, (2015) (PubMed).
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- 抗原
- Protein A
- 别名
- ProteinA (Protein A 产品)
- 别名
- alpha protein antibody, repB antibody
- 背景
- Chicken Anti-Protein A [HRP] Polyclonal Antibody is developed in chicken host animals using purified recombinant protein A. Chicken anti-Protein A [HRP] is highly purified from chicken yolks by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Chicken anti-Protein A is suitable for ELISA and immunoblotting.
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