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- 抗原 See all Protein A 抗体
- Protein A
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适用
- Staphylococcus aureus
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Protein A antibody is conjugated to HRP
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 纯化方法
- Anti-Protein A (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus].
- 免疫原
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Immunogen: Protein A [Staphylococcus aureus]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product Protein A Primary Antibody
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anti-Protein A antibody (HRP)
适用: Staphylococcus aureus ELISA, WB 宿主: 小鸡 Polyclonal HRP
anti-Protein A antibody适用: Staphylococcus aureus IP, Con 宿主: 山羊 Polyclonal unconjugated
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IP, DB 宿主: 山羊 Polyclonal unconjugated
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein A antibody (Texas Red (TR))适用: Staphylococcus aureus FACS, FM 宿主: 山羊 Polyclonal Texas Red (TR)
anti-Protein A antibody (FITC)适用: Staphylococcus aureus FACS 宿主: 山羊 Polyclonal FITC
anti-Protein A antibody (Biotin)适用: Staphylococcus aureus ELISA, WB, IHC 宿主: 山羊 Polyclonal Biotin
anti-Protein A antibody (Texas Red (TR))适用: Staphylococcus aureus FACS, IM 宿主: 山羊 Polyclonal Texas Red (TR)
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- 应用备注
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Immunohistochemistry Dilution: 1:250
Application Note: Anti-Protein A Peroxidase Conjugated has been assayed against 1.0 μg of Protein A [Staphylococcus aureus] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 is suggested for this product.
Western Blot Dilution: 1:500-1:2,000
ELISA Dilution: 1:2,000-1:20,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Protein A
- Abstract
- Protein A 产品
- 别名
- alpha protein antibody, repB antibody
- 背景
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Synonyms: HRP, Horseradish Peroxidase, Anti-Protein A HRP Antibody
Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunglobulins. Horse Raddish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Protein A (GOAT) Antibody is ideal for researchers in Immunology, Cancer, and Microbiology.
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