Collagen Type V 抗体
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- 抗原 See all Collagen Type V (COL5) 抗体
- Collagen Type V (COL5) (Collagen, Type V (COL5))
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适用
- 人, Cow, 哺乳动物
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Collagen Type V antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- Typically less than 1 % cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- 纯化方法
- Immunoaffinity Chromatography.
- 免疫原
- Collagen type V purified from Human and Bovine placenta.
- 亚型
- IgG
- Top Product
- Discover our top product COL5 Primary Antibody
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anti-Collagen, Type V (COL5) antibody
COL5 适用: 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type V (COL5) (AA 1450-1500) antibodyCOL5 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type V (COL5) antibodyCOL5 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type V (COL5) antibody (Biotin)COL5 适用: 人, Cow, 哺乳动物 ELISA, WB, IHC, IP 宿主: 兔 Polyclonal Biotin
anti-Collagen, Type V (COL5) (AA 61-90), (N-Term) antibodyCOL5 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type V (COL5) antibody (HRP)COL5 适用: 人, Cow, 哺乳动物 ELISA, WB, IHC, IP 宿主: 兔 Polyclonal HRP
anti-Collagen, Type V (COL5) antibody (FITC)COL5 适用: 人, Cow, 哺乳动物 ELISA, WB, IHC, IP 宿主: 兔 Polyclonal FITC
anti-Collagen, Type V (COL5) antibody (Biotin)COL5 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type V (COL5) antibodyCOL5 适用: 人, Cow, 哺乳动物 ELISA, WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 2.1 mg/mL (by UV absorbance at 280nm)
- 缓冲液
- 0.125 M Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide, None
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Collagen Type V (COL5) (Collagen, Type V (COL5))
- 别名
- Collagen Type V (COL5 产品)
- 背景
- Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Extensively purified collagens are used for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.Synonyms: COL5A1
- 基因ID
- 1289
- NCBI登录号
- NP_000084
- UniProt
- P20908
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