COL2A1 抗体 (Chain alpha 1)
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- 抗原 See all COL2A1 抗体
- COL2A1 (Collagen, Type II, alpha 1 (COL2A1))
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抗原表位
- Chain alpha 1
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适用
- 人, 小鼠, 大鼠, Cow, 绵羊
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This COL2A1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 特异性
- This product has been prepared by Immunoaffinity Chromatography using immobilised antigens followed by extensive cross-adsorbtion against other collagens, Human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Crossreactivity < 1 % against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- 纯化方法
- Immunoaffinity Chromatography.
- 免疫原
- Collagen type II purified from Human knee and Bovine nasal cartilage.
- Top Product
- Discover our top product COL2A1 Primary Antibody
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anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1307-1383) antibody
COL2A1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 70-150) antibodyCOL2A1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 873-1072) antibodyCOL2A1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) antibodyCOL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (N-Term) antibodyCOL2A1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1222-1241), (C-Term) antibodyCOL2A1 适用: 小鼠, 大鼠 WB, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 231-330) antibodyCOL2A1 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (C-Term) antibodyCOL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (N-Term) antibodyCOL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1143-1297) antibodyCOL2A1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide, None
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- COL2A1 (Collagen, Type II, alpha 1 (COL2A1))
- Abstract
- COL2A1 产品
- 别名
- ANFH antibody, AOM antibody, COL11A3 antibody, SEDC antibody, Col2 antibody, Col2a antibody, Col2a-1 antibody, Del1 antibody, Dmm antibody, Lpk antibody, M100856 antibody, Rgsc856 antibody, CG2A1A antibody, COLLII antibody, col2a1a antibody, CB11 antibody, col2a1 antibody, coll2a1 antibody, fb38c06 antibody, fc10c01 antibody, wu:fb38c06 antibody, wu:fc10c01 antibody, collagen type II alpha 1 chain antibody, collagen, type II, alpha 1 antibody, collagen, type II, alpha 1 L homeolog antibody, collagen, type II, alpha 1 S homeolog antibody, collagen, type II, alpha 1a antibody, COL2A1 antibody, Col2a1 antibody, col2a1 antibody, col2a1.L antibody, col2a1.S antibody, col2a1a antibody
- 背景
- Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. For immunization collagens from human and bovine placenta and cartilage extensively purified by limited pepsin digestion and selective salt precipitation were used. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.Synonyms: Alpha-1 type II collagen, COL2A1
- 基因ID
- 1280
- NCBI登录号
- NP_001835
- UniProt
- P02458
- 途径
- Sensory Perception of Sound, Growth Factor Binding
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