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COL2A1 抗体 (C-Term)

Name: COL2A1 抗体 (C-Term)
SKU: ABIN5518709
Availability: OutOfStock

COL2A1 适用: 小鼠, 大鼠 WB, IHC (fro) 宿主: 兔 Polyclonal unconjugated

Anti-Collagen II antibody, Western blotting Lane 1: Rat Heart Tissue Lysate Lane 1: Rat Brain Tissue Lysate

Anti-Collagen II antibody, IHC(F): Rat Trachea Tissue

IHC

2 images

(35 references)

产品编号 ABIN5518709

发货至: 中国

Datasheet as PDF

抗原 See all COL2A1 抗体

COL2A1 (Collagen, Type II, alpha 1 (COL2A1))
抗原表位
15

14

9

8

5

4

4

3

3

2

2

1

1

1

1

1

1

1

1

1
AA 1222-1241, C-Term
适用
66

31

29

19

6

2

2

2

1

1

1
小鼠, 大鼠
宿主
69

10

2

1
兔
Compatible Secondaries
克隆类型
73

9
多克隆
标记
49

7

6

5

2

2

1

1

1

1

1

1

1

1

1

1

1
This COL2A1 antibody is un-conjugated
应用范围
63

37

22

18

17

13

13

13

9

9

8

7

3
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

原理

Rabbit IgG polyclonal antibody for Collagen alpha-1(II) chain(COL2A1) detection. Tested with WB, IHC-F in Mouse,Rat.

序列

AFAGLGQREK GPDPMQYMRA

交叉反应（详细）

No cross reactivity with other proteins.

产品特性

Rabbit IgG polyclonal antibody for Collagen alpha-1(II) chain(COL2A1) detection. Tested with WB, IHC-F in Mouse,Rat.
Gene Name: collagen, type II, alpha 1
Protein Name: Collagen alpha-1(II) chain

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen II1222-1241aa AFAGLGQREKGPDPMQYMRA), different from the related rat sequence by one amino acid.

亚型

IgG

Top Product

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anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1307-1383) antibody
COL2A1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (AA 70-150) antibody
COL2A1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (AA 873-1072) antibody
COL2A1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) antibody
COL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (N-Term) antibody
COL2A1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (AA 231-330) antibody
COL2A1 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (C-Term) antibody
COL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (N-Term) antibody
COL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1143-1297) antibody
COL2A1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1228-1487) antibody
COL2A1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse, The detection limit for COL2A1 is approximately 1 ng/lane under reducing conditions.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(F).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Sodium azide, Thimerosal (Merthiolate)

注意事项

This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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Li, Zou, Wang, Wang, Li, Chen, Sun, Sun: "TGF-β1/Smad3 Signaling Pathway Mediates T-2 Toxin-Induced Decrease of Type II Collagen in Cultured Rat Chondrocytes." in: Toxins, Vol. 9, Issue 11, (2018) (PubMed).

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Hou, Huang, Luo, Wang, Liu, Deng, Zhang, Liu, Chen: "MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR." in: American journal of translational research, Vol. 9, Issue 11, pp. 4963-4973, (2017) (PubMed).

Tian, Zhong, Zheng, Zhang, Liu, Zhang, Liu: "Neuroleukin/Autocrine Motility Factor Receptor Pathway Promotes Proliferation of Articular Chondrocytes through Activation of AKT and Smad2/3." in: Scientific reports, Vol. 5, pp. 15101, (2016) (PubMed).

Liu, Tian, Zhou, Wang, Gou, Zhang, Wang, Shen, Zhang, Zhang: "Protective effect of calcitonin on lumbar fusion-induced adjacent-segment disc degeneration in ovariectomized rat." in: BMC musculoskeletal disorders, Vol. 16, pp. 342, (2016) (PubMed).

Tian, Zhong, Zhang, Yin, Zhang, Liu: "Microfluidics‑based optimization of neuroleukin‑mediated regulation of articular chondrocyte proliferation." in: Molecular medicine reports, Vol. 13, Issue 1, pp. 67-74, (2016) (PubMed).

Gu, Li, Li, Yu, Wang, Ding, Liu: "Oral Resveratrol Prevents Osteoarthritis Progression in C57BL/6J Mice Fed a High-Fat Diet." in: Nutrients, Vol. 8, Issue 4, pp. 233, (2016) (PubMed).

Li, Sun, Liu, Chen, Rong, Lou, Yang, Yang, Liu: "Use of a biological reactor and platelet-rich plasma for the construction of tissue-engineered bone to repair articular cartilage defects." in: Experimental and therapeutic medicine, Vol. 12, Issue 2, pp. 711-719, (2016) (PubMed).

Liu, Lu, Wu, Zheng: "Chondroprotective effects of taurine in primary cultures of human articular chondrocytes." in: The Tohoku journal of experimental medicine, Vol. 235, Issue 3, pp. 201-13, (2015) (PubMed).

Ye, Wu, Li, Li, Zheng, Liu, Ye: "Millimeter Wave Treatment Inhibits Apoptosis of Chondrocytes via Regulation Dynamic Equilibrium of Intracellular Free Ca (2+)." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 464161, (2015) (PubMed).

Cai, Zhang, Zhang, Ge, Dai, Tan, Roodrajeetsing, Cai: "Promoting Effects on Proliferation and Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Four "Kidney-Tonifying" Traditional Chinese Herbs." in: BioMed research international, Vol. 2015, pp. 792161, (2015) (PubMed).

Sun, Meng, Li, Liu, Chen: "Construction of tissue-engineered laryngeal cartilage with a hollow, semi-flared shape using poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) as a scaffold." in: Experimental and therapeutic medicine, Vol. 9, Issue 4, pp. 1482-1488, (2015) (PubMed).

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抗原

COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

别名

COL2A1 (COL2A1 产品)

别名

ANFH antibody, AOM antibody, COL11A3 antibody, SEDC antibody, Col2 antibody, Col2a antibody, Col2a-1 antibody, Del1 antibody, Dmm antibody, Lpk antibody, M100856 antibody, Rgsc856 antibody, CG2A1A antibody, COLLII antibody, col2a1a antibody, CB11 antibody, col2a1 antibody, coll2a1 antibody, fb38c06 antibody, fc10c01 antibody, wu:fb38c06 antibody, wu:fc10c01 antibody, collagen type II alpha 1 chain antibody, collagen, type II, alpha 1 antibody, collagen, type II, alpha 1 L homeolog antibody, collagen, type II, alpha 1 S homeolog antibody, collagen, type II, alpha 1a antibody, COL2A1 antibody, Col2a1 antibody, col2a1 antibody, col2a1.L antibody, col2a1.S antibody, col2a1a antibody

背景

Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Synonyms: Alpha 1 type II collagen antibody|Alpha-1 type II collagen antibody|AOM antibody|Cartilage collagen antibody|Chondrocalcin antibody|CO2A1_HUMAN antibody|COL11A3 antibody|COL2A1 antibody|COL2A1 protein antibody|Collagen alpha 1(II) chain precursor antibody|Collagen II alpha 1 polypeptide antibody|Collagen type II alpha 1(primary osteoarthritis spondyloepiphyseal dysplasia congenital) antibody|MGC131516 antibody|SEDC antibody

UniProt

P28481

途径

Sensory Perception of Sound, Growth Factor Binding