电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

RNA Polymerase 2 (AA 101-200) 抗体 (HRP)

适用: 小鼠 WB, IHC (fro), IHC (p), ELISA 宿主: 兔 Polyclonal HRP
产品编号 ABIN1415595
发货至: 中国
  • 抗原
    RNA Polymerase 2
    抗原表位
    • 16
    • 16
    • 15
    AA 101-200
    适用
    • 32
    • 16
    • 15
    小鼠
    宿主
    • 47
    克隆类型
    • 47
    多克隆
    标记
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    HRP
    应用范围
    • 47
    • 42
    • 39
    • 39
    • 8
    • 8
    • 8
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA
    交叉反应
    小鼠
    预测反应
    Human,Rat,Dog,Cow,Pig,Horse
    纯化方法
    Purified by Protein A.
    免疫原
    KLH conjugated synthetic peptide derived from human Pol II/RNA polymerase II
    亚型
    IgG
  • 应用备注
    WB 1:300-5000
    IHC-P 1:200-400
    IHC-F 1:100-500
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 μg/μL
    缓冲液
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    储存液
    ProClin
    注意事项
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    注意事项
    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    储存条件
    -20 °C
    储存方法
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    RNA Polymerase 2
    背景

    Synonyms: POLR2A, POLR2, DNA directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit, DNA-directed RNA polymerase II subunit A, RPB1_HUMAN.

    Background: RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

    基因ID
    5430
    UniProt
    P24928
You are here:
客服