RNA Polymerase 2 (AA 101-200) 抗体
(1 reference)-
- 抗原
- RNA Polymerase 2
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抗原表位
- AA 101-200
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- 小鼠
- 预测反应
- Human,Rat,Dog,Cow,Pig,Horse
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human Pol II/RNA polymerase II
- 亚型
- IgG
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anti-RNA Polymerase 2 (pSer2) antibody
适用: 人, 大鼠 WB, FACS, IF (cc), IF (p), ELISA, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase 2 (pSer5) antibody适用: 人 WB, FACS, IF (cc), IF (p), ELISA, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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: "STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells." in: Scientific reports, Vol. 6, pp. 26691, (2018) (PubMed).
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: "STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells." in: Scientific reports, Vol. 6, pp. 26691, (2018) (PubMed).
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- 抗原
- RNA Polymerase 2
- 背景
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Synonyms: POLR2A, POLR2, DNA directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit, DNA-directed RNA polymerase II subunit A, RPB1_HUMAN.
Background: RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.
- 基因ID
- 5430
- UniProt
- P24928
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