SGK1 抗体 (AA 400-431)
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- 抗原 See all SGK1 抗体
- SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))
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抗原表位
- AA 400-431
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SGK1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 200-401-844
- Supplier
- Rockland
- 原理
- SKG-1 Antibody
- 交叉反应 （详细）
- Anti-SKG-1 Antibody is directed against human SGK-1 protein.
- 产品特性
- Synonyms: rabbit anti-SKG-1 Antibody, Serum/glucocorticoid-regulated kinase 1, SGK 1 antibody, SGK1 antibody, Cellular stress response antibodies, Serine/threonine-protein kinase Sgk1
- 纯化方法
- The product was protein A purified from monospecific antiserum.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: SKG-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 400-431 of human SGK-1.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product SGK1 Primary Antibody
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anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-431) antibody
SGK1 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 4D7-G3 unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pSer422) antibodySGK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 172-431) antibodySGK1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-90) antibodySGK1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pSer78) antibodySGK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 145-386) antibodySGK1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 247-406) antibodySGK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-90) antibodySGK1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3E3 unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pSer422) antibodySGK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pThr256) antibodySGK1 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 10 μg/mL to 20 μg/mL
Application Note: SKG-1 Antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~49 kDa in size corresponding to SGK-1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:4,000 - 1:16,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 5.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store SKG-1 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))
- 别名
- SGK1 (SGK1 产品)
- 背景
- Background: SGK-1 (also called Serine/threonine-protein kinase Sgk1 and Serum/glucocorticoid-regulated kinase 1) is a protein kinase that plays an important role in cellular stress response. SGK1 activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. This kinase mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A and phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, Kv1.3, or EAAT1. SGK1 is localized to the cytoplasm and upon phosphorylation is translocated to the nucleus. The kinase is expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Induction occurs upon exposure to glucocorticoids and by excessive extracellular glucose or TGF-beta, in cultured cells. SGK-1 is regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1. The kinase is ubiquitinated by NEDD4L, which promotes proteasomal degradation.
- 基因ID
- 6446
- UniProt
- O00141
- 途径
- MAPK Pathway, Notch Signaling, Steroid Hormone Mediated Signaling Pathway
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