Caspase 9 抗体 (Asp330, N-Term)
Quick Overview for Caspase 9 抗体 (Asp330, N-Term) (ABIN127028)
抗原
See all Caspase 9 (CASP9) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Asp330, N-Term
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特异性
- The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells. The anti-active caspase-9 antibody recognizes only the cleaved caspase-9 (37 kDa). It does not recognize full-length caspase-9 or any other caspases.
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纯化方法
- Affinity chromatography
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免疫原
- Synthetic peptide mapping to the N-terminus adjacent to Asp330 of human Caspase-9
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anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (AA 1-98) antibody
CASP9 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (AA 11-120) antibodyCASP9 适用: 人, 小鼠, 大鼠, 兔, 小鸡, 犬 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (AA 331-416) antibodyCASP9 适用: 人 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (AA 1-200) antibodyCASP9 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (AA 1-200) antibodyCASP9 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (AA 1-416) antibodyCASP9 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 3B8-4G2 unconjugated
anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (AA 271-314) antibodyCASP9 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) antibodyCASP9 适用: 人 WB, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (pThr125) antibodyCASP9 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (Cleaved-Asp353) antibodyCASP9 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 0.5-4 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunohistochemistry /Immunocytochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- PBS containing 50 % glycerol, 0.5 % BSA, and 0.02 % thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at -20 °C to -70 °C.
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- Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))
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别名
- Caspase-9
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背景
- Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase-9 (47 kDa) and Apaf-1. The complex processes pro-caspase-9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved Caspase-9 further processes other caspases including caspase-3 and caspase-6, to initiate a caspase cascade leading to apoptosis.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6
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基因ID
- 842
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NCBI登录号
- NP_001220
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UniProt
- P55211
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途径
- MAPK Pathway, RTK signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity
抗原
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