This antibody specifically recognizes STAT5A phosphorylated at Tyr 694 and STAT5B phosphorylated at Tyr 699. The antibody does not crossreact with the non-phosphorylated form of STAT5 nor with unrelated tyrosine-phosphorylated proteins.
产品特性
Synonyms: STAT-5, STAT-5A, Mammary gland factor, MGF, Signal transducer and activator oftranscription 5A
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunohistochemistry. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.1mg/mL
缓冲液
PBS/0.09 % Na-Azide/PEG and Sucrose/50 % Glycerol
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody (Aliquote and freeze in liquid nitrogen) at -20 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Shelf life: one year from despatch.
有效期
12 months
抗原
STAT5A
(Signal Transducer and Activator of Transcription 5A (STAT5A))
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背景
The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT5 is activated in response to prolactine, growth hormone, GM-CSF, and IL-2.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A