STAT3 抗体 (pTyr705)
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- 抗原 See all STAT3 抗体
- STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
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抗原表位
- pTyr705
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适用
- 人, 小鼠, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This STAT3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 特异性
- The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STAT3 is activated by tyrosine phosphorylation at residue Tyr 705 in cells treated with interleukin 6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1. AM00148PU-N specifically recognizes STAT3 phosphorylated at Tyr 705 in human, mouse and dog. The antibody does not crossreact with the non-phosphorylated form of STAT3 nor with unrelated tyrosine-phosphorylated proteins. AM00148PU-N is particularly suitable for Western blot and ELISA applications.
- 产品特性
- Synonyms: STAT-3, Acute-phase response factor, APRF, Signal transducer and activator oftranscription 3
- 纯化方法
- Size exclusion chromatography
- 组件
- incl. positive Control
- 免疫原
- Synthetic phosphopeptide conjuagted to KLH
- 克隆位点
- 9-00E-012
- 亚型
- IgG1
- Top Product
- Discover our top product STAT3 Primary Antibody
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anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody
KO Validated STAT3 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibodySTAT3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pSer727) antibodySTAT3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pTyr705) antibodySTAT3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (AA 640-770) antibodySTAT3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (AA 233-330) antibodySTAT3 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pSer727) antibodySTAT3 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS, IF (cc) 宿主: 兔 Monoclonal 4G1 unconjugated
anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (AA 670-769) antibodySTAT3 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 4D6 unconjugated
anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibodyVerified STAT3 适用: 人, 小鼠 IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) antibodyKO Validated STAT3 适用: 人 WB, IHC, IP, IF 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10g per 106 pervanadate treated HepG2 cells. Immunocytochemistry: 1 - 10 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 1 mL H2O (15 min, RT).
- 缓冲液
- 1 mL2 x PBS with 0.09 % Sodium Azide, PEG and Sucrose
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C/-80 °C
- 储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
- 别名
- STAT3 (STAT3 产品)
- 别名
- 1110034C02Rik antibody, AW109958 antibody, Aprf antibody, APRF antibody, HIES antibody, Xstat3 antibody, aprf antibody, hies antibody, stat3 antibody, wu:fc15d02 antibody, wu:fl59g06 antibody, z-Stat3 antibody, signal transducer and activator of transcription 3 antibody, signal transduction and activation of transcription 3 antibody, signal transducer and activator of transcription 3, gene 1 L homeolog antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody, STAT3 antibody, stat3 antibody, Stat3 antibody, stat3.1.L antibody
- 背景
- The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT3 is activated by tyrosine phosphorylation at residue Tyr 705 in cells treated with interleukin 6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1.Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3
- 分子量
- 92 kDa
- 基因ID
- 6774
- UniProt
- P40763
- 途径
- JAK/STAT Signaling, RTK signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
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