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MEK1 抗体 (pSer218, pSer222, pSer226)

Name: MEK1 抗体 (pSer218, pSer222, pSer226)
SKU: ABIN126836
Availability: OutOfStock

The 小鼠单克隆 anti-MEK1 antibody is suitable to detect MEK1 in samples from 人, 小鼠和犬. It has been validated for WB 和 EIA.

Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer218), (pSer222), (pSer226) antibody (ABIN126836) (MEK1 抗体 (pSer218, pSer222, pSer226))

1 image

产品编号 ABIN126836

发货至: 中国

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Quick Overview for MEK1 抗体 (pSer218, pSer222, pSer226) (ABIN126836)

抗原

See all MEK1 (MAP2K1) 抗体

MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

适用

人, 小鼠, 犬

宿主

小鼠

克隆类型

单克隆

标记

This MEK1 antibody is un-conjugated

应用范围

Western Blotting (WB), Enzyme Immunoassay (EIA)

克隆位点

7-00E-010

Discover our top product MAP2K1 Primary Antibody

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pSer218, pSer222, pSer226

特异性

AM00090PU-N specifically recognizes MEK1 phosphorylated at serine 218/222 and MEK2 phosphorylated at serine 222/226.

产品特性

Synonyms: MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1, PRKMK1, MAP kinase kinase 1,Dual specificity mitogen-activated protein kinase kinase 1

纯化方法

Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

组件

incl. positive Control

免疫原

Phosphopeptide conjugated to KLH. Epitope: pSer 218/222 in MEK1 pSer 222/226 in MEK2AA Sequence: D pS M A N pS FRemarks: Mol. Weight of Antigen: 45 kDa

亚型

IgG1
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody
MAP2K1 适用: 人 ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 1-100) antibody
MAP2K1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody
MAP2K1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3H3 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody
MAP2K1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 2H3 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody
KD Validated MAP2K1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB2475 unconjugated Recombinant Antibody

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody
MAP2K1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3B4 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer221) antibody
MAP2K1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (N-Term) antibody
MAP2K1 适用: 人, 小鼠, 大鼠, 非洲爪蟾 WB, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 54-369) antibody
MAP2K1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-79) antibody
MAP2K1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

Suitable for Western Blot (0.5 μg/mL for HRPO/ECL detection)Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use SW480 pervanadate-treated as positive control (see Protocols below). ELISA: 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

实验流程

Contains Positive Control (pervanadate treated SW 480 cells)Format: Lyophilized cell lysate from SW480 cells. Serum starved cells were treated for15min with pervanadate. Reconstitution: Add 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRP/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation. Aliquot and store frozen. Avoid repeated freeze/thaw cycles.

限制

仅限研究用
溶解方式

Reconstitute with 1 mL H2O (15 min, RT)

缓冲液

1 x PBS / 0.09 % Na-azide / PEG and Sucrose

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

有效期

12 months
抗原

MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

别名

MAP2K1

背景

MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase 1 and 2 on both threonine and tyrosine residues of the activation loop motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1

基因ID

5604

UniProt

Q02750

途径

MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling