MAPK10 抗体
Quick Overview for MAPK10 抗体 (ABIN126824)
抗原
See all MAPK10 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Antibody AM00080PU-N specifically interacts with the N-terminus of SAPK1 beta/jnk3 kinase. Shows no cross-reactivity with jnk1 or jnk2
-
产品特性
- Synonyms: Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, JNK3A, PRKM10, MAPkinase p49 3F12, Mitogen-activated protein kinase 10
-
纯化方法
- Purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography
-
组件
- incl. positive Control
-
免疫原
- Peptide conjugated to hemocyaninEpitope: N-terminusRemarks: Molecular weight of antigen 54 kDa
-
亚型
- IgG
-
-
anti-Mitogen-Activated Protein Kinase 10 (MAPK10) (AA 175-464) antibody
MAPK10 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 10 (MAPK10) (AA 180-329) antibodyMAPK10 适用: 人, 大鼠 WB, ELISA 宿主: 小鼠 Monoclonal 2B10H12 unconjugated
anti-Mitogen-Activated Protein Kinase 10 (MAPK10) (AA 88-332) antibodyMAPK10 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 10 (MAPK10) (AA 28-233) antibodyMAPK10 适用: 人, 小鼠 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 10E4A4 unconjugated
anti-Mitogen-Activated Protein Kinase 10 (MAPK10) (C-Term) antibodyMAPK10 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 10 (MAPK10) (AA 1-319) antibodyMAPK10 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4E12-1F7 unconjugated
anti-Mitogen-Activated Protein Kinase 10 (MAPK10) (C-Term) antibodyMAPK10 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 10 (MAPK10) antibodyMAPK10 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 8A5D11 unconjugated
anti-Mitogen-Activated Protein Kinase 10 (MAPK10) (AA 25-74) antibodyMAPK10 适用: 人, 小鼠, 大鼠, Cow, 犬, 山羊, 豚鼠, 马, 兔, 斑马鱼, Bat, 小鸡, 猴, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 10 (MAPK10) (AA 1-30), (N-Term) antibodyMAPK10 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB01017 unconjugated
-
-
应用备注
-
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use cell lysate from untreated SH-SY5Y cells delivered with this product as positive control: see protocol below!
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Positive Control Cell Lysate: Untreated SH-SY5YFormat: Lyophilized cell lysate from serum starved SH-SY5Y cells. Reconstitute by addition of 200 µl H20. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Applications: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.
-
限制
- 仅限研究用
-
-
-
溶解方式
- Restore with 1 mL H2O (15 min, RT).
-
缓冲液
- 2 x PBS / 0.09 % Na-azide / PEG and Sucrose
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
-
For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -20 °C to -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at2-8 °C up to 3 months. Avoid repeated freeze / thaw cycles.
Shelf life: one year from despatch. -
有效期
- 12 months
-
-
- MAPK10 (Mitogen-Activated Protein Kinase 10 (MAPK10))
-
别名
- MAPK10
-
背景
- Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. The SAPK1/jnk family consists of 3 isoforms: SAPK1alpha/jnk2, SAPK1beta/jnk3, SAPK1gamma/jnk1.Synonyms: JNK3A, MAP kinase p49 3F12, Mitogen-activated protein kinase 10, PRKM10, Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3
-
基因ID
- 5602
-
UniProt
- P53779
-
途径
- MAPK Pathway, WNT signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
抗原
-
