Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
特异性
The monoclonal antibody cocktail decorates the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus. Polypeptides reacting: Lamin isotypes of Mr 60 - 75 kDa.
纯化方法
Protein A affinity chromatography
免疫原
Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells)
Western blotting. Immunofluorescence microscopy. Immunohistochemistry: dilute further 1: 10 (incubation time 1 h at room temperature). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in 1.0 mL distilled water.
缓冲液
0.09 % sodium azide
储存液
Sodium azide
注意事项
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Nuclear Lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type Lamins and B-type Lamins. The A-type Lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. Lamin A, Lamin C and Lamin Adel 10, while the B-type Lamins include two proteins arising from two distinct genes, i.e. Lamin B1 and Lamin B2. Recent evidence has revealed that mutations in A-type Lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type Lamins coincides with cell differentiation and as A-type Lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type Lamins.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32