Nestin 抗体 (AA 1484-1500)
Our Local Distributor
北京 101111
Quick Overview for Nestin 抗体 (AA 1484-1500) (ABIN117995)
抗原
See all Nestin (NES) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 1484-1500
-
特异性
- This antibody detects Nestin protein.
-
交叉反应 （详细）
-
Species reactivity (expected):A BLAST analysis was used to suggest cross reactivity with Nestin protein from Human and Macaque (100 % homology). Based on protein sequence homology also expect partial reactivity against Nestin homologues from Rabbit (94 %), Mouse (reactivity shown above), Rat and Hamster (88 %), Bovine and Dog (82 %).
Species reactivity (tested):Human. -
纯化方法
- Immunoaffinity Chromatography.
-
免疫原
- A recombinant protein corresponding to amino acids 1484-1500 of Human Nestin protein.
-
-
anti-Nestin (NES) (AA 1389-1416) antibody
NES 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB16253 unconjugated
anti-Nestin (NES) (AA 178-399) antibodyNES 适用: 人 IHC, WB, ICC, IP 宿主: 小鼠 Monoclonal D10 unconjugated
anti-Nestin (NES) antibodyNES 适用: 人 IHC, WB, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 2C2F7 unconjugated
anti-Nestin (NES) (AA 252-290), (N-Term) antibodyNES 适用: 人, 小鼠, 大鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) antibodyNES 适用: 小鼠 IHC, ICC 宿主: 大鼠 Monoclonal 7A3 unconjugated
anti-Nestin (NES) (AA 600-900) antibodyNES 适用: 人 IHC 宿主: 小鼠 Monoclonal NES-2911 unconjugated
anti-Nestin (NES) (AA 1484-1500) antibodyNES 适用: 人, 小鼠 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 IHC, WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 681-750) antibodyNES 适用: 人, 小鼠, 大鼠 ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
This antibody has been tested for use in ELISA and Western blot. Expect a bandapproximately 200-220 kDa in size corresponding to Nestin protein by Western blotting inthe appropriate cell lysate or extract. Recommended Dilutions: ELISA: 1/20,000-1/80,000. Western blot: 1/500-1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 1.1 mg/mL (by UV absorbance at 280 nm)
-
缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, with 0.01 % (w/v) Sodium Azide as preservative.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store undiluted at 2-8 °C.
-
-
- Nestin (NES)
-
别名
- Nestin
-
背景
- Nestin is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker, however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.Synonyms: NES, Nbla00170
-
基因ID
- 10763
-
NCBI登录号
- NP_006608
-
UniProt
- P48681
抗原
-
