-
- 抗原 See all CD28 抗体
- CD28
-
适用
- 大鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This CD28 antibody is un-conjugated
-
应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP)
- 品牌
- BD Pharmingen™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- 免疫原
- Rat Cd28-transfected BALB/c mouse A20J B lymphoma
- 克隆位点
- JJ316
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CD28 Primary Antibody
-
-
- 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
-
-
CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." in: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, (1997) (PubMed).
: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).
: "Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, ..." in: European journal of immunology, Vol. 25, Issue 2, pp. 328-32, (1995) (PubMed).
: "
-
CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." in: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, (1997) (PubMed).
-
- 抗原
- CD28
- 别名
- CD28 (CD28 产品)
- 别名
- Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody
- 背景
- The JJ316 antibody reacts with CD28 on virtually all T lymphocytes bearing alphabetaT-cell receptors (TCR), on most gammadeltaTCR-bearing T cells, and on a subset of NK cells. In the thymus, CD28 expression is developmentally regulated during the maturation of alphabetaTCR-bearing T cells. CD28 is a costimulatory receptor required for activation of T cells, its ligands include CD80 (B7-1) and CD86 (B7-2). Soluble JJ316 enhances the proliferative responses and IL-2 production by T cells activated by anti-aß-TCR mAb R73 (Cat. no. 554910). Furthermore, at high concentrations, cross-linked JJ316 mAb stimulates resting CD4+ and CD8+T cells to proliferate, secrete IL-2, express activation markers, and gain CTL activity in the absence of signaling through the TCR. In vivo administration of mAb JJ316 results in blastogenic transformation of peripheral CD4+ cells and dramatic lymphocytosis. The alternate anti-rat CD28 mAb JJ319 (Cat. no. 554993) cannot directly activate T cells, although it has in vitro costimulatory activity.
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
-