(2 references)-
- 抗原 See all IL-10RA (IL10RA) 抗体
- IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL-10RA antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Neutralization (Neut)
- 品牌
- BD Pharmingen™
- 产品特性
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of the activity elicited by recombinant mouse IL-10. Neutralization activity may be measured with a proliferation assay with 1x10^5 cells/mL D36 indicator cells using 6 ng/mL recombinant mouse IL-10 (Cat. No. 550070) costimulated with 50 pg/mL recombinant mouse IL-4 (Cat. No. 550067) (i.e preincubatiion of the antibody with the cells for 60 min)
50% Neutralization (ND50) at 0.1 - 1 ng/mL
>95% Neutralization at ≥ 1 - 15 ng/mL - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- 免疫原
- Purified recombinant ligand-binding domain of mIL-10R
- 克隆位点
- 1B1-3a
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product IL10RA Primary Antibody
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anti-Interleukin 10 Receptor, alpha (IL10RA) (AA 132-235) antibody
IL10RA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (AA 22-230) antibodyIL10RA 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) antibodyIL10RA 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (C-Term) antibodyIL10RA 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (pTyr496) antibodyIL10RA 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (Ser560) antibodyIL10RA 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (AA 128-177) antibodyIL10RA 适用: 人, Cow, Pig, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) antibodyIL10RA 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (AA 147-175) antibodyIL10RA 适用: 人 WB, FACS, IF 宿主: 兔 Polyclonal RB23343 unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (AA 81-180) antibodyIL10RA 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways." in: The EMBO journal, Vol. 17, Issue 4, pp. 1006-18, (1998) (PubMed).
: "A sensitive and specific bioassay for the detection of human interleukin-10." in: Journal of immunological methods, Vol. 168, Issue 1, pp. 49-54, (1994) (PubMed).
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: "IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways." in: The EMBO journal, Vol. 17, Issue 4, pp. 1006-18, (1998) (PubMed).
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- 抗原
- IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))
- 别名
- CD210 (IL10RA 产品)
- 别名
- IL10R1 antibody, IL10RA antibody, CD210 antibody, CD210a antibody, CDW210A antibody, HIL-10R antibody, IL-10R1 antibody, IL10R antibody, AW553859 antibody, CDw210 antibody, CDw210a antibody, Il10r antibody, mIL-10R antibody, interleukin 10 receptor subunit alpha antibody, interleukin 10 receptor, alpha antibody, IL10RA antibody, Il10ra antibody
- 背景
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The 1B1.3a antibody reacts with the extracellular region of mouse CD210 which is also known as the mouse IL-10 receptor (mIL-10R), it does not recognize the human IL-10R. The IL-10R is expressed by a variety of mouse cell types and cell lines including thymocytes, T cells, B cells, and monocytes. 1B1.3a is a neutralizing antibody and reportedly blocks the binding of human IL-10, which cross-reacts with the mouse IL-10R. The immunogen used for the generation of the 1B1.3a hybridoma was purified recombinant ligand-binding domain of mIL-10R.
Synonyms: IL-10 Receptor - 途径
- Growth Factor Binding
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