STAT1 抗体 (pSer727) (Alexa Fluor 647)
(2 references)-
- 抗原 See all STAT1 抗体
- STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))
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抗原表位
- pSer727
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This STAT1 antibody is conjugated to Alexa Fluor 647
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应用范围
- Intracellular Staining (ICS)
- 品牌
- BD Phosflow™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Phosphorylated Human Stat1 Peptide
- 克隆位点
- K51-856
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product STAT1 Primary Antibody
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anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody
STAT1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 343-602) antibodySTAT1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibodySTAT1 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) antibodySTAT1 适用: 人, 犬, 猴 WB, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 4H9 unconjugated
anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 717-745), (C-Term) antibodySTAT1 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB41406 unconjugated
anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 1-712) antibodySTAT1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 650-750) antibodySTAT1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 513-712) antibodySTAT1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 1-712) antibodySTAT1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 613-712) antibodySTAT1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1A8 unconjugated
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- 应用备注
- Either BD Cytofix™ fixation buffer or BD Phosflow™ Fix Buffer I may be used for cell fixation. Any of the three BD Phosflow™ permeabilization buffers may be used.
- 样本量
- 20 μL
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "STAT-1 interacts with p53 to enhance DNA damage-induced apoptosis." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5811-20, (2004) (PubMed).
: "Phosphorylation of the Stat1 transactivation domain is required for full-fledged IFN-gamma-dependent innate immunity." in: Immunity, Vol. 19, Issue 6, pp. 793-802, (2003) (PubMed).
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: "STAT-1 interacts with p53 to enhance DNA damage-induced apoptosis." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5811-20, (2004) (PubMed).
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- 抗原
- STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))
- 别名
- Stat1 (STAT1 产品)
- 别名
- CANDF7 antibody, ISGF-3 antibody, STAT91 antibody, 2010005J02Rik antibody, AA408197 antibody, XStat1 antibody, STAT4 antibody, DD6G4-4 antibody, STAT antibody, STAT1 antibody, dZ182H3.3 antibody, si:by134g18.2 antibody, si:by51f19.1 antibody, si:dz182h3.3 antibody, si:dz199m19.1 antibody, si:dz73o4.2 antibody, stat1 antibody, signal transducer and activator of transcription 1 antibody, signal transducer and activator of transcription 1 L homeolog antibody, signal transducer and activator of transcription 1, 91kDa antibody, signal transducer and activator of transcription 4 antibody, signal transducer and activator of transcription 1a antibody, STAT1 antibody, Stat1 antibody, stat1.L antibody, stat4 antibody, stat1a antibody
- 背景
- Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84-kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs). Furthermore, phosphorylation of serine 727 (S727) of Stat1 may occur in response to pathogens, cytokines, UV irradiation, and engagement of T and B cell antigen receptors. This second phosphorylation event is necessary for fully effective activation of transcription and may differentially regulate gene activation by interacting with other regulatory factors, such as p53 in the apoptotic pathway. The K51-856 monoclonal antibody recognizes the phosphorylated S727 in the C-terminal transactivating domain of human Stat1alpha, this site is not present in Stat1beta.
- 途径
- JAK/STAT Signaling, RTK signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events
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