(4 references)-
- 抗原 See all IL-10 (IL10) 抗体
- IL-10 (IL10) (Interleukin 10 (IL10))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL-10 antibody is un-conjugated
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应用范围
- ELISA, Neutralization (Neut)
- 品牌
- BD Pharmingen™
- 产品特性
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-10. Neutralization activity may be measured with a proliferation assay using 5 ng/mL recombinant mouse IL-10 (Cat. No. 550070) and 0.1 ng/mL recombinant mouse IL-4 (Cat. No. 550067) to co-stimulate MC/9 cells at 1x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with the recombinant mouse IL-10 for 60 min).
50% Neutralization (ND50) at 2 - 8 ng/mL
>95% Neutralization 3 - 25 µg/mL - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- 免疫原
- Mouse IL-10
- 克隆位点
- JES5-2A5
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product IL10 Primary Antibody
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anti-Interleukin 10 (IL10) (AA 101-178) antibody
IL10 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 19-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 19-178) antibodyIL10 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) antibodyIL10 适用: 人, 小鼠, 大鼠, Cow, 兔, 马, Pig, 犬, 猴, 小鸡, 绵羊, 山羊, Avian, Cat, 驴, 豚鼠, Hamster WB 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 10 (IL10) antibodyMicroarray verified IL10 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB971550 unconjugated
anti-Interleukin 10 (IL10) antibodyMicroarray verified IL10 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB312926 unconjugated
anti-Interleukin 10 (IL10) antibodyIL10 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Interleukin 10 (IL10) (AA 27-53) antibodyIL10 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) antibodyIL10 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 1-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- ELISA: The purified format of the JES5-2A5 antibody (Cat. No. 551215) has been reported to be useful as a capture antibody in sandwich ELISA for mouse IL-10. Purified JES5-2A5 antibody can be paired with the biotinylated SXC-1 antibody (Cat. No. 554423) or biotinylated JES5-16E3 antibody (Cat. No. 554465) and recombinant mouse IL-10 (Cat. No. 550070) as the standard. Purified JES5-2A5 antibody should be titrated (suggested 2-6 µg/mL) to determine the optimal concentration for ELISA capture. An appropriate range of concentrations of the mouse IL-10 standard for obtaining a linear standard curve is 2 ng/mL to 15 pg/mL mouse IL-10.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
: "Induction of cytokines in mice with parainfluenza pneumonia." in: Journal of virology, Vol. 69, Issue 2, pp. 1288-91, (1995) (PubMed).
: "Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1246-53, (1994) (PubMed).
: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).
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: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
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- 抗原
- IL-10 (IL10) (Interleukin 10 (IL10))
- 别名
- IL-10 (IL10 产品)
- 别名
- CSIF antibody, GVHDS antibody, IL-10 antibody, IL10A antibody, TGIF antibody, Il-10 antibody, IL10X antibody, interleukin 10 antibody, IL10 antibody, Il10 antibody
- 背景
- The JES5-2A5 antibody reacts with mouse IL-10. The immunogen used to generate the JES5-2A5 hybridoma was E. coli-expressed mouse IL-10. This is a neutralizing antibody.
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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