COL2A1 抗体 (Chain alpha 1) (Biotin)
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- 抗原 See all COL2A1 抗体
- COL2A1 (Collagen, Type II, alpha 1 (COL2A1))
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抗原表位
- Chain alpha 1
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适用
- 人, 小鼠, 大鼠, Cow, 绵羊
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This COL2A1 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 特异性
- Crossreactivity < 1 % to other collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- 交叉反应 （详细）
- Species reactivity (tested):Human, Mouse, Rat, Bovine, Sheep.
- 产品特性
- Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
- 纯化方法
- Immunoaffinity Chromatography using immobilised antigens followed by extensive cross-adsorbtion against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
- 免疫原
- Collagen type II purified from adult Human knee and Bovine nasal cartilage. For immunization collagens extensively purified by limited pepsin digestion and selective salt precipitation were used. This preparation results in a native conformation of the protein.
- Top Product
- Discover our top product COL2A1 Primary Antibody
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anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1307-1383) antibody
COL2A1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 70-150) antibodyCOL2A1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 873-1072) antibodyCOL2A1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) antibodyCOL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (N-Term) antibodyCOL2A1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1222-1241), (C-Term) antibodyCOL2A1 适用: 小鼠, 大鼠 WB, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 231-330) antibodyCOL2A1 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (C-Term) antibodyCOL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (N-Term) antibodyCOL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1143-1297) antibodyCOL2A1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended dilutions: ELISA 1: 10,000 - 1: 50,000Western blot 1: 5,000 - 1: 10,000Immunohistochemistry 1: 200 - 1: 1,000
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.1 M Sodium Borate, 0.071 M Sodium Chloride, 0.001 M EDTA, pH 8.0, 0.01 % Sodium Azide, 10 mg/mL BSA (IgG and Protease free)
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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- 抗原
- COL2A1 (Collagen, Type II, alpha 1 (COL2A1))
- Abstract
- COL2A1 产品
- 别名
- ANFH antibody, AOM antibody, COL11A3 antibody, SEDC antibody, Col2 antibody, Col2a antibody, Col2a-1 antibody, Del1 antibody, Dmm antibody, Lpk antibody, M100856 antibody, Rgsc856 antibody, CG2A1A antibody, COLLII antibody, col2a1a antibody, CB11 antibody, col2a1 antibody, coll2a1 antibody, fb38c06 antibody, fc10c01 antibody, wu:fb38c06 antibody, wu:fc10c01 antibody, collagen type II alpha 1 chain antibody, collagen, type II, alpha 1 antibody, collagen, type II, alpha 1 L homeolog antibody, collagen, type II, alpha 1 S homeolog antibody, collagen, type II, alpha 1a antibody, COL2A1 antibody, Col2a1 antibody, col2a1 antibody, col2a1.L antibody, col2a1.S antibody, col2a1a antibody
- 背景
- Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.Synonyms: Alpha-1 type II collagen, COL2A1
- 基因ID
- 1280
- NCBI登录号
- NP_001835
- UniProt
- P02458
- 途径
- Sensory Perception of Sound, Growth Factor Binding
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