Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFGF-10 (1.5 ng/mL), a concentration of 0.02-0.035 μg/mL of this antibody is required. ELISA: (Indirect): To detect hFGF-10 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hFGF-10. (Sandwich): To detect hFGF-10 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human FGF-10 (PP1119B1 or PP1119B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinanthFGF-10. Western Blot: To detect hFGF-10 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-10 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry on frozen or paraffin sections: This antibody should be used at0.5-2.0 μg/mL depending on dection system. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
FGF10 is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. FGF10 is mostly related to FGF-7/KGF and is expressed during development and preferentially in adult lungs.Synonyms: FGF-10, Fibroblast growth factor 10, KGF2, Keratinocyte growth factor 2