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IL-10 抗体 (Biotin)

IL10 适用: 人 WB, EIA 宿主: 兔 Polyclonal Biotin
产品编号 ABIN116098
发货至: 中国
  • 抗原 See all IL-10 (IL10) 抗体
    IL-10 (IL10) (Interleukin 10 (IL10))
    适用
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    克隆类型
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    多克隆
    标记
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    This IL-10 antibody is conjugated to Biotin
    应用范围
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    Western Blotting (WB), Enzyme Immunoassay (EIA)
    纯化方法
    Affinity chromatography.
    免疫原
    Highly pure (>98%) recombinant hIL-10 (human IL-10).
    Top Product
    Discover our top product IL10 Primary Antibody
  • 应用备注
    ELISA: To detect hIL-10 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-10. Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-10 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原
    IL-10 (IL10) (Interleukin 10 (IL10))
    别名
    Interleukin-10 / IL10 (IL10 产品)
    别名
    CSIF antibody, GVHDS antibody, IL-10 antibody, IL10A antibody, TGIF antibody, Il-10 antibody, IL10X antibody, interleukin 10 antibody, IL10 antibody, Il10 antibody
    背景
    Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73 % homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.Synonyms: CSIF, Cytokine synthesis inhibitory factor, IL-10, TGIF
    基因ID
    9606
    UniProt
    P22301
    途径
    Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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