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IL-6 抗体 (Biotin)

IL6 适用: 大鼠 WB, EIA 宿主: 兔 Polyclonal Biotin
产品编号 ABIN115959
发货至: 中国
  • 抗原 See all IL-6 (IL6) 抗体
    IL-6 (IL6) (Interleukin 6 (IL6))
    适用
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    大鼠
    宿主
    • 193
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    • 50
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    克隆类型
    • 213
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    多克隆
    标记
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    This IL-6 antibody is conjugated to Biotin
    应用范围
    • 266
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    Western Blotting (WB), Enzyme Immunoassay (EIA)
    特异性
    This antibody recognises Rat Interleukin-6 (rIL-6)
    纯化方法
    Affinity chromatography.
    免疫原
    Highly pure (>98%) recombinant rat IL-6.
    Top Product
    Discover our top product IL6 Primary Antibody
  • 应用备注
    ELISA: To detect rIL-6 by direct ELISA (using 100 l/well antibody solution) this antibody can beused at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant rIL-6. Western Blot: To detect rIL-6 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant rIL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives.
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原
    IL-6 (IL6) (Interleukin 6 (IL6))
    别名
    Interleukin-6 (IL6) (IL6 产品)
    背景
    Interleukin 6 (IL6) is a multifunctional 24 kD protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass. IL6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL6 appears to interact with IL2 in the proliferation of T lymphocytes. IL6 potentiates the proliferative effect of IL3 on multipotential hematopoietic progenitors.Synonyms: B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFNB2, IL-6, Interferon beta-2
    基因ID
    24498
    NCBI登录号
    NP_036721
    UniProt
    P20607
    途径
    TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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