Collagen IV 抗体
Quick Overview for Collagen IV 抗体 (ABIN114114)
抗原
See all Collagen IV (COL4) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Antibody BP975 is specific for Mouse collagen type IV. The following cross reactivities have been observed: Mouse Type IV: 100 % Mouse Type III: < 0.1 % Mouse Type II: < 0.1 % Mouse Type I: < 0.1 % Human Type V: < 0.1 % Human Type IV: < 0.1 % Mouse Fibronectin: < 0.1 % Mouse Laminin: < 0.1 %
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交叉反应 （详细）
- Species reactivity (expected):OrangutanSpecies reactivity (tested):Mouse
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纯化方法
- Antigen Affinity Chromatography
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免疫原
- Collagen IV purified from Mouse EHS tumour
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anti-Collagen, Type IV (COL4) antibody
COL4 适用: 人, Cow WB, ELISA, IHC, IP, DB, FM 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1571-1669) antibodyCOL4 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1401-1500) antibodyCOL4 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA, IHC, IHC (fro), IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-704R unconjugated Recombinant Antibody
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人, 大鼠, Pig, Cow WB, IHC, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal LP46 unconjugated
anti-Collagen, Type IV (COL4) antibody (FITC)COL4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F11 FITC
anti-Collagen, Type IV (COL4) antibody (Biotin)COL4 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
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应用备注
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Immunohistochemistry on Paraffin Embedded Sections (1/250-1/1,000): Antigen retrievalis not necessary but may improve results, the recommended fixatives are AFA, 5-10 % buffered formalin or Bouin's. Immunofluorescence (1/40-1/60). ELISA (1/100-1/400).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store undiluted (in aliquots) at -20 °C.
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- Collagen IV (COL4) (Collagen, Type IV (COL4))
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别名
- Collagen Type IV
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背景
- Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.Synonyms: COL4A1
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基因ID
- 12826
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NCBI登录号
- NP_034061
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UniProt
- P02463
抗原
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