This antibody is specific for Collagen type II. Cross-reactivity: Human Collagen type I and IV: < 1 % Human Collagen type III, IX and XI: < 0.1 %. Human Fibronectin: < 0.1 % (RIA: 1/200).
ELISA. RIA. Immunofluorescence: 1/40. Immunohistochemistry: 1/200-1/600 for Paraffin Sections, 60 min at RT or 2-8 °C overnight. Positive Control: Human skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
限制
仅限研究用
溶解方式
Restore with 0.5 mL distilled water, for further dilution use appropriate antibody diluent.
浓度
1.0 mg/mL
缓冲液
PBS without additives.
储存液
Without preservative
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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背景
Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type II collagen is an alpha1(II)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and various tumours.Synonyms: Alpha-1 type II collagen, COL2A1