COL2A1 抗体 (Chain alpha 1)
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- 抗原 See all COL2A1 抗体
- COL2A1 (Collagen, Type II, alpha 1 (COL2A1))
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抗原表位
- Chain alpha 1
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This COL2A1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)
- 特异性
- Murine Procollagen and Collagen type II: 100 % . Murine Collagen type I, III and IV < 0.1 %. Mouse Fibronectin: < 0.1 %. Cross-reacts with Bovine Collagen type II in ELISA.
- 交叉反应 （详细）
- Species reactivity (tested):Mouse.
- 纯化方法
- Affinity Chromatography.
- 免疫原
- Purified Collagen type II from Fetal Murine Cartilage.
- Top Product
- Discover our top product COL2A1 Primary Antibody
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anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1307-1383) antibody
COL2A1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 70-150) antibodyCOL2A1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 873-1072) antibodyCOL2A1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) antibodyCOL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (N-Term) antibodyCOL2A1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1222-1241), (C-Term) antibodyCOL2A1 适用: 小鼠, 大鼠 WB, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 231-330) antibodyCOL2A1 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (C-Term) antibodyCOL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (N-Term) antibodyCOL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1143-1297) antibodyCOL2A1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1/200 (OD >/= 500). Radioimmunoassay. Immunofluorescence: 1/80. Immunohistochemistry (Parrafin): 1/1000 (60 min at RT or 2-8 °C over night). Positive Control: Mouse Cartilage. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water, for further dilution use appropriate antibody diluent.
- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS without BSA and preservatives
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- COL2A1 (Collagen, Type II, alpha 1 (COL2A1))
- Abstract
- COL2A1 产品
- 别名
- ANFH antibody, AOM antibody, COL11A3 antibody, SEDC antibody, Col2 antibody, Col2a antibody, Col2a-1 antibody, Del1 antibody, Dmm antibody, Lpk antibody, M100856 antibody, Rgsc856 antibody, CG2A1A antibody, COLLII antibody, col2a1a antibody, CB11 antibody, col2a1 antibody, coll2a1 antibody, fb38c06 antibody, fc10c01 antibody, wu:fb38c06 antibody, wu:fc10c01 antibody, collagen type II alpha 1 chain antibody, collagen, type II, alpha 1 antibody, collagen, type II, alpha 1 L homeolog antibody, collagen, type II, alpha 1 S homeolog antibody, collagen, type II, alpha 1a antibody, COL2A1 antibody, Col2a1 antibody, col2a1 antibody, col2a1.L antibody, col2a1.S antibody, col2a1a antibody
- 背景
- Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type II collagen is an alpha1(II)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and various tumours.Synonyms: Alpha-1 type II collagen, COL2A1
- 基因ID
- 12824
- NCBI登录号
- NP_001106987
- UniProt
- P28481
- 途径
- Sensory Perception of Sound, Growth Factor Binding
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