COL3A1 抗体 (Chain alpha 1)
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- 抗原 See all COL3A1 抗体
- COL3A1 (Collagen, Type III, alpha 1 (COL3A1))
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抗原表位
- Chain alpha 1
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This COL3A1 antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)
- 特异性
- Human Collagen type III 100 % (solid phase RIA at 1/500 dilution). Type III Collagen, MW 95 kDa, can be observed in skin, cartilage, human placenta and vitreous body. Cross Reactions: Human Collagen I, II, IV and V: < 0,1 % Human Fibronectin and Laminin: < 0,1 %. Does not react with Mouse, Rat, Bovine and Pig.
- 纯化方法
- Affinity Chromatography.
- 免疫原
- Purified collagen type III from human placenta
- 克隆位点
- 8D1-8C7
- 亚型
- IgG1
- Top Product
- Discover our top product COL3A1 Primary Antibody
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anti-Collagen, Type III, alpha 1 (COL3A1) (C-Term) antibody
COL3A1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III, alpha 1 (COL3A1) (AA 1217-1466) antibodyCOL3A1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III, alpha 1 (COL3A1) (AA 24-153) antibodyCOL3A1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 8C1G11 unconjugated
anti-Collagen, Type III, alpha 1 (COL3A1) (AA 1171-1435) antibodyCOL3A1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III, alpha 1 (COL3A1) (AA 1059-1466) antibodyCOL3A1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III, alpha 1 (COL3A1) antibodyCOL3A1 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III, alpha 1 (COL3A1) antibodyCOL3A1 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 5G2 unconjugated
anti-Collagen, Type III, alpha 1 (COL3A1) (N-Term) antibodyCOL3A1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III, alpha 1 (COL3A1) (AA 1171-1435) antibodyCOL3A1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Collagen, Type III, alpha 1 (COL3A1) (N-Term) antibodyCOL3A1 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 兔 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- ELISA. RIA (> 1/1000). Immunflourescence assay: 1/80Immunohistochemistry on Paraffin Sections: 1/1000 (60 min at RT or 2-8 °C over night). Immunohistochemistry on Frozen Sections. Pretreatment for IHC: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding isblocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 %peroxide solution in TBS for 30 min at RT is recommended. Positive Control: Human skin and placenta.
- 限制
- 仅限研究用
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- 溶解方式
- Restore by adding 0.1 mL distilled water.
- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS without preservatives or BSA.
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions
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- 抗原
- COL3A1 (Collagen, Type III, alpha 1 (COL3A1))
- Abstract
- COL3A1 产品
- 别名
- EDS4A antibody, AW550625 antibody, Col3a-1 antibody, MMS10-W antibody, Ms10w antibody, mKIAA4231 antibody, col3a1 antibody, collagen type III alpha 1 chain antibody, collagen, type III, alpha 1 antibody, collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant) S homeolog antibody, COL3A1 antibody, Col3a1 antibody, col3a1 antibody, col3a1.S antibody
- 背景
- Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage, vitreous body, and human placenta.Synonyms: COL3A1
- 基因ID
- 1281
- NCBI登录号
- NP_000081
- UniProt
- P02461
- 途径
- Autophagy, Growth Factor Binding
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