BrdU 抗体
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- 抗原 See all BrdU 抗体
- BrdU (Bromodeoxyuridine (BrdU))
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This BrdU antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 纯化方法
- Purified
- 免疫原
- IIB5 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse intraperitoneally immunized with BrdU conjugated to BSA
- 克隆位点
- IIB5
- 亚型
- IgG1
- Top Product
- Discover our top product BrdU Primary Antibody
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anti-Bromodeoxyuridine (BrdU) antibody
BrdU IHC (p), IF (p), IF (cc), IHC (fro), WB 宿主: 小鼠 Monoclonal A7 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, FACS, WB, ELISA, FM 宿主: 小鼠 Monoclonal 29G6-E8 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (p), IF (cc), IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal MoBu-1 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 小鼠 Monoclonal BRD469 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 小鼠 Monoclonal 4B6C12 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 兔 Monoclonal BRD2888R unconjugated Recombinant Antibody
anti-Bromodeoxyuridine (BrdU) antibody (PE)BrdU FACS 宿主: 小鼠 Monoclonal Bu20a PE
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, FACS, IF, Neut, StM 宿主: 小鼠 Monoclonal SPM166 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU ELISA, Coat 宿主: 兔 Monoclonal BRD-1539R unconjugated Recombinant Antibody
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- 应用备注
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This antibody is suitable for Flow cytometry and Immunohistochemistry on frozen andparaffin-embedded tissues. Recommended dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the lyophilized antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. When reconstituted the antibody is stable for one month at 2-8 °C.
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- 抗原
- BrdU (Bromodeoxyuridine (BrdU))
- Abstract
- BrdU 产品
- 物质类
- Chemical
- 背景
- The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
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