Lamin A/C 抗体
Quick Overview for Lamin A/C 抗体 (ABIN112042)
抗原
See all Lamin A/C (LMNA) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody 133A2 recognizes an epitope located between residues 598-611 of Lamin A, therefore it reacts exclusively with Lamin A.
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纯化方法
- Purified
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免疫原
- Partially purified recombinant Human Lamin A.
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亚型
- IgG3
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anti-Lamin A/C (LMNA) (AA 403-572) antibody
LMNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Lamin A/C (LMNA) antibodyKO Validated LMNA 适用: 人 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lamin A/C (LMNA) (pSer392) antibodyLMNA 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lamin A/C (LMNA) antibodyKO Validated LMNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Lamin A/C (LMNA) antibodyLMNA 适用: 人 IHC, ELISA, IF, IP, FACS 宿主: 兔 Monoclonal 4H7 unconjugated Recombinant Antibody
anti-Lamin A/C (LMNA) antibodyLMNA 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4E7 unconjugated
anti-Lamin A/C (LMNA) (AA 481-646) antibodyLMNA 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lamin A/C (LMNA) (AA 455-469) antibodyLMNA 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lamin A/C (LMNA) antibodyLMNA 适用: 人, 小鼠, 大鼠, Cow, 犬 WB, IHC, ICC, FACS, IHC (fro) 宿主: 小鼠 Monoclonal 133A2 unconjugated
anti-Lamin A/C (LMNA) (C-Term) antibodyLMNA 适用: 人, 小鼠, 大鼠, Cow, Pig, 犬, 猴, 兔, 马, Hamster, 小鸡, 绵羊, 豚鼠, Avian, Cat, 驴, 山羊 WB 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Immunocytochemistry. Immunohistochemistry on frozen sections (1: 100 - 1: 200 with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent). Immunoblotting (1: 100 - 1: 1000). Flow cytometry (1: 100 - 1: 200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Immunofluorescence protocol - Formaldehyde fixation1. Collect cells from T. c. unit and remove media from petri dish using suction. 2. Wash with 1x PBS and remove. 3. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at roomtemperature on an orbital shaker. 4. Remove PFA and incubate in 0. 5% Triton X-IOO in 1x PBS for 5 minutes at roomtemperature. 5. Prepare blocking reagent, this is also the antibody diluent. 6. Wash cells 2x with 1x PBS at room temperature, for 4 minutes/wash on an orbital shaker. 7. Block with 1 % NCS and 1x PBS for 30 minutes at room temperature. 8. Prepare primary antibodies (50μl/coverslip) and moist staining chambers. 9. Wash cells 2x with lx PBS at room temperature and air dry briefly.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS containing 0.09 % Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Lamin A/C (LMNA)
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别名
- Lamin-A/C (LMNA)
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背景
- Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32
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分子量
- 74 kDa (Predicted)
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基因ID
- 4000
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NCBI登录号
- NP_005563
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UniProt
- P02545
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途径
- Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus
抗原
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