ITGA5 抗体
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- 抗原 See all ITGA5 抗体
- ITGA5 (Integrin, alpha 5 (ITGA5))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ITGA5 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Detects human granulocytes, monocytes and platelets. X-6 detects an antigen of 154-180 kDa by immunoprecipitation. Antigen distribution on Isolated cells and Tissue sections: Granulocytes, monocytes. Positive on blood monocyte-derived macrophages days 0-28. Dull staining with 95 % cord blood CD34 cells. Weak reaction on resting neutrophils. Staining increase with maturation of granulocytes. Treatment with fMLP, TNF, GM-CSF and temperature causes postive shift with granulocytes and monocytes. PMA treatment of granulocytes leads to decrease in no. of positive cells. X-6 is positive on lining cells in different infiltrations and endothelial cells in the synovial membrane of RA patients. The staining pattern is similar to CD34 in these patients.
- 纯化方法
- Affinity chromatography.
- 免疫原
- Human monocytes.
- 克隆位点
- X-6
- 亚型
- IgG1
- Top Product
- Discover our top product ITGA5 Primary Antibody
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anti-Integrin, alpha 5 (ITGA5) (AA 42-263) antibody
ITGA5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 5 (ITGA5) (AA 1021-1099) antibodyITGA5 适用: 人, 小鼠 WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 5 (ITGA5) antibodyITGA5 适用: 人 WB, FACS, ELISA, IHC 宿主: 小鼠 Monoclonal 10F6 unconjugated
anti-Integrin, alpha 5 (ITGA5) (AA 111-253) antibodyITGA5 适用: 人 WB, FACS, ELISA, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 8D1G12 unconjugated
anti-Integrin, alpha 5 (ITGA5) (AA 111-253) antibodyITGA5 适用: 人 WB, FACS, ELISA, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 6F6C1 unconjugated
anti-Integrin, alpha 5 (ITGA5) (AA 220-472) antibodyITGA5 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 5 (ITGA5) (Internal Region) antibodyITGA5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 5 (ITGA5) (AA 750-1000) antibodyITGA5 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 5 (ITGA5) (AA 575-602) antibodyITGA5 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB20677 unconjugated
anti-Integrin, alpha 5 (ITGA5) (Internal Region) antibodyITGA5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Suitable for Immunohistochemistry on frozen sections (2 μg/mL (1: 100) and FACS (use 10 μLstock solution to label 10^6 cells).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % Thimerosal as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
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- 抗原
- ITGA5 (Integrin, alpha 5 (ITGA5))
- 别名
- CD49e / ITGA5 (ITGA5 产品)
- 别名
- ITGA5 antibody, Cd49e antibody, Fnra antibody, VLA5 antibody, CD49e antibody, FNRA antibody, VLA5A antibody, Itga5_retired antibody, integrin subunit alpha 5 antibody, integrin alpha 5 (fibronectin receptor alpha) antibody, ITGA5 antibody, Itga5 antibody
- 背景
- The X-6 antibody is useful for the detection of the fibronectin receptor, or CD49e. CD49e is a component of an adhesion receptor heterodimer (α5β1) to fibronectin and invasin. α5β1 recognizes an Arg-Gly-Asp sequence in fibronectin. Binding of fibronectin through α5β1 is important for cell survival and induces apoptosis, particularly in hemopoietic cells.Synonyms: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5
- 基因ID
- 3678
- NCBI登录号
- NP_002196
- UniProt
- P08648
- 途径
- Integrin Complex
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