MBP Tag 抗体
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- 抗原
- MBP Tag
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宿主
- 小鼠
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标记
- 非结合性
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应用范围
- Western Blotting (WB)
- 免疫原
- Recombinant MBP protein with full length.
- 亚型
- IgG1
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anti-MBP Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-MBP Tag antibodyWB 宿主: 兔 Polyclonal RB55937 unconjugated
anti-MBP Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 3G1A3 unconjugated
anti-MBP Tag antibodyWB 宿主: 小鼠 Monoclonal 8K2 unconjugated
anti-MBP Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 5B10B11 unconjugated
anti-MBP Tag antibodyWB, EIA 宿主: 小鼠 Monoclonal 6-00E-004 unconjugated
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- 应用备注
- WB (1:1000-10,000). Other applications have not been tested. The optimal dilutions should be determined by end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
- 储存液
- Sodium azide, Thimerosal (Merthiolate)
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C for 1 month. Or aliquot and store at -20 °C or below for long term. Avoid repeated freeze and thaw cycles.
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- 抗原
- MBP Tag
- 物质类
- Tag
- 背景
- Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It has an approximate molecular mass of 42.5 kilodaltons. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose.
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