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- 抗原
- MBP Tag
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This antibody can be used for the detection of MBP fusion proteins.
- 产品特性
- Synonyms: MMBP, Maltodextrin-binding protein, malE-Tag
- 纯化方法
- Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
- 免疫原
- MBP (Maltose Binding Protein)
- 克隆位点
- 6-00E-004
- 亚型
- IgG1
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anti-MBP Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-MBP Tag antibodyWB 宿主: 兔 Polyclonal RB55937 unconjugated
anti-MBP Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 3G1A3 unconjugated
anti-MBP Tag antibodyWB 宿主: 小鼠 Monoclonal 8K2 unconjugated
anti-MBP Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 5B10B11 unconjugated
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- 应用备注
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Western blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 1 mL H2O (15 min, RT)
- 缓冲液
- 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C/-80 °C
- 储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- MBP Tag
- 别名
- Maltose Binding Protein Tag / MBP-Tag
- 物质类
- Tag
- 背景
- Maltose Binding Protein (MBP), the E. coli malE gene product, is widely used as fusion partner for the expression of recombinant proteins.Synonyms: MMBP, Maltodextrin-binding protein, malE-Tag
- 分子量
- 43 kDa
- 基因ID
- 948538
- UniProt
- P0AEX9
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