TCR beta 抗体 (APC)
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- 抗原 See all TCR beta 抗体
- TCR beta (T Cell Receptor (TCR) beta (TCR beta))
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适用
- 小鼠
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宿主
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Armenian Hamster
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克隆类型
- 单克隆
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标记
- This TCR beta antibody is conjugated to APC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 免疫原
- Affinity purified TCR from mouse DO-11.10 cells Species
- 克隆位点
- H57-597
- 亚型
- IgG
- Top Product
- Discover our top product TCR beta Primary Antibody
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anti-T Cell Receptor (TCR) beta (TCR beta) antibody (FITC)
TCR beta 适用: 小鼠 FACS 宿主: Armenian Hamster Monoclonal H57-597 FITC
anti-T Cell Receptor (TCR) beta (TCR beta) antibody (APC)TCR beta 适用: 小鼠 FACS 宿主: Armenian Hamster Monoclonal H57-597 APC
anti-T Cell Receptor (TCR) beta (TCR beta) (Internal Region) antibodyTCR beta 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-T Cell Receptor (TCR) beta (TCR beta) antibodyTCR beta 适用: 小鼠 FACS, IP 宿主: 仓鼠 Monoclonal H57-597 unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using 0,25 µg/10^6 cells
- 说明
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Allophycocyanin (APC). Abs/Em: 651/662 nm.
- 试剂准备
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- Transfer the sample to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse anti-mouse TCR beta APC and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- TCR beta (T Cell Receptor (TCR) beta (TCR beta))
- 别名
- TCR beta (TCR beta 产品)
- 别名
- TCRbeta antibody, Tib antibody, T cell receptor beta chain antibody, Tcrb antibody
- 背景
- T cell receptor (TCR) is a heterodimer consisting of an alpha and a beta chain (TCR alpha/beta) or a gamma and a delta chain (TCR gamma/delta). TCR-beta is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-alpha). It is expressed on alpha/beta TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.
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