CD5 抗体 (PerCP)
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- 抗原 See all CD5 抗体
- CD5
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD5 antibody is conjugated to PerCP
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Rat monoclonal Anti-Mouse CD5 PerCP is recommended for use in flow cytometry for identification of the thymocytes, T cells, and B-1 cells.
- 免疫原
- Mouse thymus or spleen
- 克隆位点
- 53-7-3
- 亚型
- IgG2a
- Top Product
- Discover our top product CD5 Primary Antibody
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anti-CD5 (CD5) (AA 105-210) antibody
CD5 适用: 人 WB 宿主: 山羊 Polyclonal unconjugated
anti-CD5 (CD5) (AA 269-366) antibodyCD5 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal CD5-2416 unconjugated
anti-CD5 (CD5) (AA 25-372) antibodyCD5 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD5 (CD5) (AA 269-366) antibodyCD5 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal CD5-2418 unconjugated
anti-CD5 (CD5) antibodyCD5 适用: 人 FACS, WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal L17F12 unconjugated
anti-CD5 (CD5) (AA 27-233) antibodyCD5 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 6A11 unconjugated
anti-CD5 (CD5) (AA 403-495) antibodyCD5 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 6C3B2 unconjugated
anti-CD5 (CD5) (AA 403-495) antibodyCD5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F7 unconjugated
anti-CD5 (CD5) antibodyCD5 适用: 人 FACS, WB, IP, ELISA, IHC (fro) 宿主: 小鼠 Monoclonal CRIS1 unconjugated
anti-CD5 (CD5) antibodyCD5 适用: 人 FACS, WB, IP, ELISA, IHC (p) 宿主: 小鼠 Monoclonal MEM-32 unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using less than or equal to less than or equal to 0,25 µg/10^6 cells.
- 说明
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PerCP. Abs/Em: 482/ 678 nm.
- 试剂准备
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- Transfer the sample of blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse CD5 PerCP and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive PerCP-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- CD5
- 别名
- CD5 (CD5 产品)
- 别名
- CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody
- 背景
- CD5 is a 67 kDa protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature alpha/beta T cells express high levels of CD5, very few gamma/epsilon T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.
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