CD55 抗体 (PE)
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- 抗原 See all CD55 抗体
- CD55 (Complement Decay-Accelerating Factor (CD55))
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适用
- 小鼠
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宿主
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Armenian Hamster
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克隆类型
- 单克隆
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标记
- This CD55 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- The anti-Mouse DAF monoclonal antibody detects DAF; one of several membrane proteins that prevent host cells from homologous complement attack. The two mouse DAF isoforms are effective against rat complement, but not against human and guinea pig complement. GPI-DAF is expressed only in testis. Both types of DAF transfectans avoided C3 deposition more successfully than non-transfectant cells.
- 免疫原
- GPI-DAF transfected CHO cells. Species
- 克隆位点
- RIKO-3
- 亚型
- IgG
- Top Product
- Discover our top product CD55 Primary Antibody
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anti-Complement Decay-Accelerating Factor (CD55) (AA 35-134) antibody
CD55 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 1G3 unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (AA 35-285) antibodyCD55 适用: 人 WB, ELISA, IHC (fro), ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (AA 40-300) antibodyCD55 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (AA 51-79) antibodyCD55 适用: 人 WB, FACS, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (AA 35-134) antibodyCD55 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 1D7 unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (Center) antibodyCD55 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (AA 254-372) antibodyCD55 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (AA 35-126) antibodyCD55 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Decay-Accelerating Factor (CD55) antibodyCD55 适用: 人 WB, IHC, ELISA 宿主: 兔 Monoclonal 1A5 unconjugated Recombinant Antibody
anti-Complement Decay-Accelerating Factor (CD55) antibodyCD55 适用: 人 FACS, IF 宿主: 小鼠 Monoclonal F4-29D9 unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using less than or equal to 0,25 µg/10^6 cells
- 说明
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R-Phycoerythrin (R-PE). Abs/Em: 545/575 nm.
- 试剂准备
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- Transfer the sample of blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse CD55 PE and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. CD55 recognizes the erythrocytes, so if you want a white blood cell labeling will be required prior lyse with ammonium chloride. 3. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- CD55 (Complement Decay-Accelerating Factor (CD55))
- 别名
- CD55 (CD55 产品)
- 别名
- CR antibody, CROM antibody, DAF antibody, TC antibody, Daf antibody, Daf-GPI antibody, Daf1 antibody, GPI-DAF antibody, CD55 molecule (Cromer blood group) antibody, CD55 molecule, decay accelerating factor for complement antibody, CD55 antibody, Cd55 antibody
- 背景
- CD55, also known as DAF (decay accelerating factor), is expressed on the surfaces of red blood cells, hematopoietic cells and many non-hemotopietic cells. There are two isoforms of mouse CD55, a GPI-anchored isoform and a transmembrane isoform. Both are recognized by RIKO-3. The function of CD55 is to protect damage by complement deposition
- 途径
- Complement System, Regulation of Leukocyte Mediated Immunity
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