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SLC3A2 抗体 (FITC)

SLC3A2 适用: 人 FACS, IF 宿主: 小鼠 Monoclonal FG1-8 FITC
产品编号 ABIN1112235
发货至: 中国
  • 抗原 See all SLC3A2 抗体
    SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This SLC3A2 antibody is conjugated to FITC
    应用范围
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    Flow Cytometry (FACS), Immunofluorescence (IF)
    产品特性
    Monoclonal Mouse Anti-Human CD98 FITC is recommended for use in flow cytometry for identification of human leukocyte lineages.
    克隆位点
    FG1-8
    亚型
    IgG1
    Top Product
    Discover our top product SLC3A2 Primary Antibody
  • 应用备注
    It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
    说明

    Fluorescein isothiocyanate (Molecular Probes).

    样品收集
    1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD98 FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
    储存液
    Sodium azide
    注意事项
    1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
    储存条件
    4 °C
  • 抗原
    SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))
    别名
    CD98 (SLC3A2 产品)
    别名
    4F2 antibody, 4F2HC antibody, 4T2HC antibody, CD98 antibody, CD98HC antibody, MDU1 antibody, NACAE antibody, Mdu1 antibody, MGC53951 antibody, slc3a2 antibody, MGC53965 antibody, SLC3A2 antibody, AI314110 antibody, Cd98 antibody, Ly-10 antibody, Ly-m10 antibody, Ly10 antibody, Mgp-2hc antibody, fc65h12 antibody, wu:fc65h12 antibody, id:ibd1327 antibody, sb:cb519 antibody, wu:fe14a07 antibody, wu:fq18d12 antibody, zgc:55813 antibody, solute carrier family 3 member 2 antibody, solute carrier family 3 (amino acid transporter heavy chain), member 2 L homeolog antibody, solute carrier family 3 (amino acid transporter heavy chain), member 2 S homeolog antibody, solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 antibody, solute carrier family 3 (amino acid transporter heavy chain), member 2a antibody, solute carrier family 3 (amino acid transporter heavy chain), member 2b antibody, SLC3A2 antibody, Slc3a2 antibody, slc3a2.L antibody, slc3a2.S antibody, slc3a2a antibody, slc3a2b antibody
    背景
    This antibody recognizes the 125 kDa heterodimeric membrane glycoprotein that is constituted by an 85 kDa heavy chain and a 40 kDa light chain. CD98 is up-regulated on leukocytes and in inflammatory lesions, and strongly expressed by neoplastic cells. However, expression is not hematopoietic specific.
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