Reactive with the N-terminal domain of Human Transferrin. Inhibits proliferation of Con-A stimulated blood lymphocytes at 10-6 M. Epitope is resistant to paraffin embedding.
交叉反应 (详细)
Species reactivity (tested):Human.
纯化方法
Affinity Chromatography on Protein A
免疫原
Purified native Human Transferrin. Remarks: Antigen Modification: N-Terminus
ELISA: 1/500-1/5000. Functional Assays (Removal of Sodium Azide is recommended prior to use). Immunohistochemistry on Paraffin Sections: 10 μg/mL. Requires antigen retrieval using heat treatment prior to staining of paraffin sections(Sodium Citrate buffer pH 6.0 is recommended for this purpose). Positive Contrrol: Placental villi. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Transferrin (TF) is a glycoprotein with an approximate molecular weight of 76.5 kDa. TF is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds 1 ion of ferric iron. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, transferrin may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.Synonyms: Beta-1 metal-binding globulin, Serotransferrin, Siderophilin