His 标记 抗体

ABIN1105145 产品详细信息, 供应商: Log in to see
抗原
适用
标记
624
28
15
5
4
1
宿主
小鼠
413
189
27
23
8
8
3
2
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
58
46
37
17
13
13
10
10
10
9
9
9
8
8
8
7
7
7
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4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
应用范围
Western Blotting (WB)
569
406
165
109
83
82
79
27
13
11
8
7
6
6
5
5
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4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
选项
Supplier
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Supplier Product No.
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免疫原 Synthetic peptide 6 His
序列 HHHHHH
克隆位点 6C4
亚型 IgG1
纯化方法 Protein A agarose
别名 6xHistidine Epitope Tag
物质类 Tag
背景 A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The His-Tag fusion protein expression system is commonly used because the 6 x His-Tag on the recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
缓冲液 PBS containing 50 % glycerol, pH 7.2, without preservatives
储存液 Without preservative
注意事项 Avoid repeated freezing and thawing.
储存条件 -20 °C
储存方法 Store (in aliquots) at -20 °C.
厂商提供的图像
Western Blotting (WB) image for anti-His Tag antibody (ABIN1105145) anti-His Tag antibody
背景 Porath: "Immobilized metal ion affinity chromatography." in: Protein expression and purification, Vol. 3, Issue 4, pp. 263-81, 1992 (PubMed).

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