Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
交叉反应 (详细)
Cross reactivity against Phospho-enol-Pyruvate Carboxylase from other tissues and species may occur but have not been specifically determined.
纯度
Anti-Phospho Enol Pyruvate Carboxylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize].
内毒素水平
Low Endotoxin : No
免疫原
Anti-Phospho Enol Pyruvate Carboxylase Antibody was produced by repeated immunizations with maize leaves Phospho-enol-pyruvate Carboxylase. Immunogen Type: Native Protein
PCK1
适用: 人
ELISA, IHC, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
Anti-Phospho Enol Pyruvate Carboxylase antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below. ELISA Dilution: 1:10.000 - 1:40.000 Western Blot Dilution: 1:1.000 - 1:4.000
限制
仅限研究用
状态
Liquid
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
注意事项
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
储存条件
-20 °C
储存方法
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
有效期
Expiration date is one (1) year from date of opening.
Anti-Phospho Enol Pyruvate Carboxylase antibody detects PEP. Phosphoenolpyruvate carboxylase is an enzyme in the family of carboxy-lyases that catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form the four-carbon compound oxaloacetate. This reaction is used for carbon fixation in so-called Synonyms: PEPC 1 antibody, PEPCase 1 antibody, Phosphoenolpyruvate carboxylase 1 antibody