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PCK1 抗体 (Middle Region)

Name: PCK1 抗体 (Middle Region)
SKU: ABIN2777602
Availability: OutOfStock

PCK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, Pig, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated

Host: Rabbit Target Name: PCK1 Sample Type: Human Fetal Muscle Antibody Dilution: 1. (PCK1 抗体 (Middle Region))

Host: Rabbit Target Name: PCK1 Sample Type: Human Fetal Liver Antibody Dilution: 1. (PCK1 抗体 (Middle Region))

WB Suggested Anti-PCK1 Antibody Positive Control: Lane 1: 80ug pig serum protein Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:500 Submitted by: Martina Ondrovics, University of Veterinary Medicine Vienna (PCK1 抗体 (Middle Region))

WB Suggested Anti-PCK1 Antibody Titration: 0. (PCK1 抗体 (Middle Region))

Host: Rabbit Target Name: PCK1 Sample Type: Human Fetal Heart Antibody Dilution: 1. (PCK1 抗体 (Middle Region))

Host: Rabbit Target Name: PCK1 Sample Type: Human Adult Placenta Antibody Dilution: 1. (PCK1 抗体 (Middle Region))

Host: Rabbit Target Name: PCK1 Sample Type: MCF7 Antibody Dilution: 1. (PCK1 抗体 (Middle Region))

Host: Rabbit Target Name: PCK1 Sample Type: Human Fetal Lung Antibody Dilution: 1. (PCK1 抗体 (Middle Region))

WB Suggested Anti-PCK1 Antibody Positive Control: Lane 1: 80ug pig serum Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:1000 Submitted by: Martina Ondrovics, University of Veterinary Medicine Vienna (PCK1 抗体 (Middle Region))

Host: Rabbit Target Name: PCK1 Sample Tissue: Human MCF7 Whole Cell Antibody Dilution: 1ug/ml (PCK1 抗体 (Middle Region))

10 images

(1 reference)

产品编号 ABIN2777602

发货至: 中国

Datasheet as PDF

抗原 See all PCK1 抗体

PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
抗原表位
15

9

8

4

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1
Middle Region
适用
72

53

28

8

5

4

4

3

3

3

2

1

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠, 犬, Cow, 兔, 马, Pig, 豚鼠
宿主
86

9

2

2
兔
Compatible Secondaries
克隆类型
83

16
多克隆
标记
46

9

6

6

3

3

3

3

3

3

2

2

2

2

2

1

1

1

1
This PCK1 antibody is un-conjugated
应用范围
82

38

27

15

14

13

10

9

4

3

1

1

1

1
Western Blotting (WB)

序列

NGFFGVAPGT SVKTNPNAIK TIQKNTIFTN VAETSDGGVY WEGIDEPLAS

预测反应

Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 86%, Rat: 100%

产品特性

This is a rabbit polyclonal antibody against PCK1. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the middle region of human PCK1

Top Product

Discover our top product PCK1 Primary Antibody
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 592-622), (C-Term) antibody
PCK1 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF 宿主: 兔 Polyclonal RB03646 unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) antibody
PCK1 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 3E4 unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (C-Term) antibody
PCK1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 312-563) antibody
PCK1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Middle Region) antibody
PCK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, Pig, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) antibody
PCK1 适用: 人, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (C-Term) antibody
PCK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 330-379) antibody
PCK1 适用: 人, 小鼠, 大鼠, Cow, 兔, 马, 猴, Hamster, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) antibody
PCK1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 311-558) antibody
PCK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 622 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Perrone, Mattocks, Plummer, Chittur, Mohney, Vignola, Orentreich, Orentreich: "Genomic and metabolic responses to methionine-restricted and methionine-restricted, cysteine-supplemented diets in Fischer 344 rat inguinal adipose tissue, liver and quadriceps muscle." in: Journal of nutrigenetics and nutrigenomics, Vol. 5, Issue 3, pp. 132-57, (2012) (PubMed).
抗原

PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

别名

PCK1 (PCK1 产品)

别名

PEPCK-C antibody, PEPCK1 antibody, PEPCKC antibody, PEPCK antibody, PEPCK-M antibody, PEPCK2 antibody, 1810010O14Rik antibody, 9130022B02Rik antibody, cb856 antibody, cb924 antibody, fj93h11 antibody, zgc:63869 antibody, wu:fc51c05 antibody, wu:fj93h11 antibody, pepck-c antibody, pepck1 antibody, pepckc antibody, PCK1 antibody, PPCK1 antibody, AI265463 antibody, Pck-1 antibody, GTP antibody, PCK antibody, Pepck antibody, RATPEPCK antibody, Ppc1C antibody, PHOSPHOENOLPYRUVATE CARBOXYKINASE antibody, T28I19.150 antibody, T28I19_150 antibody, phosphoenolpyruvate carboxykinase 1 antibody, 143299_at antibody, CG10924 antibody, CG17725 antibody, Dmel\\CG17725 antibody, Dromel_CG17725_FBtr0086701_pepck_mORF antibody, dPEPCK antibody, pepck antibody, PEPC antibody, PEPCase antibody, ppc antibody, phosphoenolpyruvate carboxykinase 1 antibody, phosphoenolpyruvate carboxykinase 2, mitochondrial antibody, phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody, phosphoenolpyruvate carboxykinase 1 (soluble) antibody, phosphoenolpyruvate carboxykinase 1 S homeolog antibody, phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody, phosphoenolpyruvate carboxykinase 1, cytosolic antibody, phosphoenolpyruvate carboxylase 7 antibody, Phosphoenolpyruvate carboxykinase antibody, phosphoenolpyruvate carboxylase antibody, PCK1 antibody, PCK2 antibody, Pck2 antibody, pck1 antibody, pck1.S antibody, LOC100634531 antibody, Pck1 antibody, pep7 antibody, Pepck antibody, LOC107777405 antibody

背景

PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet.This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MGC22652, PEPCK-C, PEPCK1, PEPCKC
Protein Interaction Partner: UBC, FBXO25, C1R, APP, CUL5, BAG6, TCP1, TCEB1, SKP1, RPS15A, RPS6, RPS3A, RPS3, RPLP0, RPL4, PSMD12, PSMD2, PSMC5, PSMC4, PSMC2, PSMC1, PSMB7, PSMB4, PSMB1, PSMA5, PSMA2, PCK1, LDHA, HSPD1, HSPA1L, DNAJA1, HLA-C, HLA-B, GNAS, GNAI1, GNA12, FASN, EP300, T
Protein Size: 622

分子量

69 kDa

基因ID

5105

NCBI登录号

NM_002591, NP_002582

UniProt

P35558

途径

Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis