-
- 抗原 See all WNT2 抗体
- WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))
-
抗原表位
- Internal Region
-
适用
- 人, 猴
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This WNT2 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human WNT2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey (100%) Marmoset, Mouse, Hamster, Guinea pig, Platypus (87%) Galago, Rat, Ferret, Elephant, Panda, Dog, Cat, Bat, Horse, Rabbit, Pig, Turkey, Chicken, Armadillo (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 15 amino acid peptide from internal region of human WNT2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey (100%), Marmoset, Mouse, Hamster, Guinea pig, Platypus (87%), Galago, Rat, Ferret, Elephant, Panda, Dog, Cat, Bat, Horse, Rabbit, Pig, Turkey, Chicken, Armadillo (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product WNT2 Primary Antibody
-
anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 221-320) antibody
WNT2 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 26-360) antibodyWNT2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) antibodyWNT2 适用: 人, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 1-100) antibodyWNT2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 26-360) antibodyWNT2 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 254-287) antibodyWNT2 适用: 人 WB 宿主: 兔 Polyclonal RB49732 unconjugated
anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 143-192) antibodyWNT2 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 猴, 兔, 绵羊, 斑马鱼, Bat, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (Internal Region) antibodyWNT2 适用: 人, 猴, Chimpanzee, Gorilla, Orang-Utan IHC (p) 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (10 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))
- 别名
- WNT2 / IRP (WNT2 产品)
- 别名
- CG1916 antibody, D-wnt-2 antibody, DWnt-2 antibody, DWnt2 antibody, Dm DWnt2 antibody, Dm-2 antibody, Dmel\\CG1916 antibody, Dwnt-2 antibody, Wnt antibody, Wnt-2 antibody, dWnt2 antibody, wnt2 antibody, WNT2 antibody, irp antibody, Xwnt2 antibody, wnt-2 antibody, Xwnt-2 antibody, int1l1 antibody, 2610510E18Rik antibody, Int1l1 antibody, Irp antibody, Mirp antibody, Wnt2a antibody, INT1L1 antibody, IRP antibody, ZfWnt2 antibody, Wnt oncogene analog 2 antibody, Wnt family member 2 antibody, wingless-type MMTV integration site family member 2 antibody, wingless-type MMTV integration site family, member 2 antibody, Wnt2 antibody, wnt2 antibody, WNT2 antibody
- 背景
-
Name/Gene ID: WNT2
Family: WNT
Synonyms: WNT2, IRP, Int-1-like protein 1, Int-1-related protein, Protein Wnt-2, Secreted growth factor, INT1L1 - 基因ID
- 7472
- 途径
- WNT signaling
-
You are here:
