WNT10B 抗体 (Internal Region)
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- 抗原 See all WNT10B 抗体
- WNT10B (Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B))
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抗原表位
- Internal Region
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适用
- 人, 小鼠, Pig, 大鼠, 犬, Bat, Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This WNT10B antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human WNT10B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Dog, Bat, Pig (100%) Marmoset, Elephant, Panda, Horse, Rabbit (93%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 15 amino acid peptide from internal region of human WNT10B. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Dog, Bat, Pig (100%), Marmoset, Elephant, Panda, Horse, Rabbit (93%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product WNT10B Primary Antibody
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anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) antibody
WNT10B 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) (AA 193-222) antibodyWNT10B 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) antibodyWNT10B 适用: 人 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 5A7 unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) (AA 201-300) antibodyWNT10B 适用: 人, 小鼠, Pig WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) (AA 30-322) antibodyWNT10B 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) (AA 251-300) antibodyWNT10B 适用: 人, 小鼠, Pig, 大鼠, 犬, 马, 兔, Bat, Cow, 豚鼠, 猴, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) (AA 193-222) antibodyWNT10B 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB19661 unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) antibodyWNT10B 适用: 人, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) (Internal Region) antibodyWNT10B 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) (Internal Region) antibodyWNT10B 适用: 人, 小鼠, Pig, 大鼠, 犬, 马, 兔, Bat, 猴, Gorilla, Orang-Utan IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- WNT10B (Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B))
- 别名
- WNT10B (WNT10B 产品)
- 别名
- WNT10B antibody, Xwnt-10b antibody, Xwnt10b antibody, wnt-12 antibody, Wnt12 antibody, SHFM6 antibody, WNT-12 antibody, Xwnt-10 antibody, wnt10 antibody, wnt10-A antibody, Zwnt[d] antibody, wnt10br antibody, wnt[d] antibody, Wnt family member 10B antibody, wingless-type MMTV integration site family, member 10B antibody, Wnt family member 10B S homeolog antibody, wingless-type MMTV integration site family, member 10b antibody, WNT10B antibody, wnt10b antibody, Wnt10b antibody, wnt10b.S antibody
- 背景
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Name/Gene ID: WNT10B
Family: WNT
Synonyms: WNT10B, Protein Wnt-10b, SHFM6, WNT-12, WNT-10B protein, WNT12, Protein Wnt-12 - 基因ID
- 7480
- 途径
- WNT signaling, Skeletal Muscle Fiber Development
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