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- 抗原 See all SCN9A 抗体
- SCN9A (Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SCN9A antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human SCN9A / Nav1.7. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except SCN8A (71 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Mouse, Panda, Dog, Bovine, Horse, Rabbit, Pig (94%) Rat, Hamster, Bat (88%) Elephant, Platypus (82%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 17 amino acid peptide from internal region of human SCN9A / Nav1.7. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Mouse, Panda, Dog, Bovine, Horse, Rabbit, Pig (94%), Rat, Hamster, Bat (88%), Elephant, Platypus (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product SCN9A Primary Antibody
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anti-Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 1751-1946) antibody
SCN9A 适用: 人 IHC, WB, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal N68-6 (Formerly S68-6) unconjugated
anti-Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 1751-1946) antibody (HRP)SCN9A 适用: 人 IHC, WB, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal N68-6 (Formerly S68-6) HRP
anti-Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 1751-1946) antibody (FITC)SCN9A 适用: 人 IHC, WB, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal N68-6 (Formerly S68-6) FITC
anti-Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 1751-1946) antibody (Biotin)SCN9A 适用: 人 IHC, WB, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal N68-6 (Formerly S68-6) Biotin
anti-Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 1751-1946) antibody (PE)SCN9A 适用: 人 IHC, WB, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal N68-6 (Formerly S68-6) PE
anti-Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 1751-1946) antibody (APC)SCN9A 适用: 人 IHC, WB, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal N68-6 (Formerly S68-6) APC
anti-Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 1751-1946) antibody (PerCP)SCN9A 适用: 人 IHC, WB, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal N68-6 (Formerly S68-6) PerCP
anti-Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 1751-1946) antibody (Atto 488)SCN9A 适用: 人 IHC, WB, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal N68-6 (Formerly S68-6) Atto 488
anti-Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 1751-1946) antibody (Atto 594)SCN9A 适用: 人 IHC, WB, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal N68-6 (Formerly S68-6) Atto 594
anti-Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A) (AA 1751-1946) antibody (Atto 390)SCN9A 适用: 人 IHC, WB, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal N68-6 (Formerly S68-6) Atto 390
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- 应用备注
- Approved: IHC, IHC-P (10 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- SCN9A (Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A))
- 别名
- SCN9A / Nav1.7 (SCN9A 产品)
- 别名
- SCN9A antibody, DKFZp459G084 antibody, Nav1.7 antibody, PN1 antibody, mKIAA4197 antibody, Scn2a antibody, ETHA antibody, FEB3B antibody, GEFSP7 antibody, NE-NA antibody, NENA antibody, SFNP antibody, sodium channel, voltage-gated, type IX, alpha subunit antibody, sodium voltage-gated channel alpha subunit 9 antibody, sodium channel protein type 9 subunit alpha antibody, sodium channel, voltage-gated, type IX, alpha antibody, SCN9A antibody, LOC100016648 antibody, Scn9a antibody
- 背景
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Name/Gene ID: SCN9A
Subfamily: Sodium channel - voltage-gated
Family: Ion Channel
Synonyms: SCN9A, ETHA, GEFSP7, Nav1.7, HNE-Na, Peripheral sodium channel 1, PN1, SFNP, Sodium channel 25, Neuroendocrine sodium channel, FEB3B, NE-NA, NENA - 基因ID
- 6335
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