PTPRM 抗体 (Internal Region)
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- 抗原 See all PTPRM 抗体
- PTPRM (Protein Tyrosine Phosphatase, Receptor Type, M (PTPRM))
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 大鼠, 兔, Cow, 马, Hamster, 猴, Bat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PTPRM antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human PTPRM. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PTPRK (50 % ), PTPRT (50 %).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Bovine, Bat, Horse, Rabbit (100%) Opossum, Chicken (94%) Elephant, Panda, Dog (88%) Gorilla, Xenopus (81%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from internal region of human PTPRM. Percent identity with other species by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Bovine, Bat, Horse, Rabbit (100%), Opossum, Chicken (94%), Elephant, Panda, Dog (88%), Gorilla, Xenopus (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product PTPRM Primary Antibody
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anti-Protein Tyrosine Phosphatase, Receptor Type, M (PTPRM) (AA 565-742) antibody
PTPRM 适用: 人 ELISA, IHC, WB, ICC, FACS, Neut 宿主: 小鼠 Monoclonal 8F1C10 unconjugated
anti-Protein Tyrosine Phosphatase, Receptor Type, M (PTPRM) (AA 1197-1403) antibodyPTPRM 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Tyrosine Phosphatase, Receptor Type, M (PTPRM) antibodyPTPRM 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Tyrosine Phosphatase, Receptor Type, M (PTPRM) (Internal Region) antibodyVerified PTPRM 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Protein Tyrosine Phosphatase, Receptor Type, M (PTPRM) (AA 381-479) antibodyPTPRM 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Tyrosine Phosphatase, Receptor Type, M (PTPRM) antibodyPTPRM 适用: 人, 小鼠, 大鼠, 兔, 马, Hamster IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Tyrosine Phosphatase, Receptor Type, M (PTPRM) antibodyPTPRM 适用: 人, 兔, Cow, 犬, 猴, Gorilla, Orang-Utan IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (12 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- PTPRM (Protein Tyrosine Phosphatase, Receptor Type, M (PTPRM))
- 别名
- PTPRM / PTP mu (PTPRM 产品)
- 别名
- cb984 antibody, ptprm antibody, zgc:110218 antibody, PTPRM antibody, p-ptp-mu antibody, ptprl1 antibody, r-ptpu antibody, rptpm antibody, rptpu antibody, PTPRL1 antibody, R-PTP-MU antibody, RPTPM antibody, RPTPU antibody, hR-PTPu antibody, RPTPmu antibody, mKIAA4044 antibody, protein tyrosine phosphatase, receptor type, M, a antibody, protein tyrosine phosphatase, receptor type M antibody, protein tyrosine phosphatase, receptor type, M, b antibody, receptor-type tyrosine-protein phosphatase mu antibody, protein tyrosine phosphatase, receptor type, M antibody, ptprma antibody, PTPRM antibody, ptprmb antibody, ptprm antibody, LOC100550127 antibody, LOC100564083 antibody, Ptprm antibody
- 背景
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Name/Gene ID: PTPRM
Subfamily: R2A
Family: Protein Phosphatase
Synonyms: PTPRM, HR-PTPu, PTP mu, PTPRL1, RPTPM, Ptpmu, R-PTP-MU, RPTPU - 基因ID
- 5797
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