TRKB 抗体 (Cytoplasmic Domain)
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- 抗原 See all TRKB (NTRK2) 抗体
- TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))
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抗原表位
- Cytoplasmic Domain
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适用
- 人, 猴, 兔, 马
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TRKB antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NTRK2 / TRKB. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Horse, Rabbit (100%) Bovine (92%) Galago, Bat (83%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 12 amino acid peptide from cytoplasmic domain of human NTRK2 / TRKB. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Horse, Rabbit (100%), Bovine (92%), Galago, Bat (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NTRK2 Primary Antibody
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anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (AA 401-500) antibody
NTRK2 适用: 人, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (pTyr706) antibodyNTRK2 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (pTyr516) antibodyNTRK2 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (AA 32-282) antibodyNTRK2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (AA 207-339) antibodyNTRK2 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 10B6C4 unconjugated
anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (AA 57-70), (Extracellular Domain) antibodyNTRK2 适用: 人, 大鼠, 小鼠 WB, IHC, IF, FACS, ICC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (pTyr817) antibodyNTRK2 适用: 人, 小鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 2A1 unconjugated
anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) antibodyNTRK2 适用: 人, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (AA 1-477) antibodyNTRK2 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (C-Term) antibodyNTRK2 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (20 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))
- 别名
- NTRK2 / TRKB (NTRK2 产品)
- 别名
- trkb antibody, trkb-b antibody, xtrkb antibody, si:ch211-220b11.1 antibody, trkB2 antibody, NTRK2 antibody, ntrk2 antibody, gp145-trkb antibody, TRKB antibody, GP145-TrkB antibody, trk-B antibody, AI848316 antibody, C030027L06Rik antibody, Tkrb antibody, trkB antibody, RATTRKB1 antibody, TRKB1 antibody, Trk-B antibody, TrkB antibody, neurotrophic receptor tyrosine kinase 2 antibody, neurotrophic receptor tyrosine kinase 2 L homeolog antibody, neurotrophic tyrosine kinase, receptor, type 2b antibody, neurotrophic receptor tyrosine kinase 2 S homeolog antibody, neurotrophic tyrosine kinase, receptor, type 2 antibody, NTRK2 antibody, ntrk2.L antibody, ntrk2b antibody, ntrk2.S antibody, ntrk2 antibody, Ntrk2 antibody
- 背景
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Name/Gene ID: NTRK2
Subfamily: Trk/Ror
Family: Protein Kinase
Synonyms: NTRK2, gp145-TrkB, Trk-B, TRKB, Tropomyosin-related kinase B, TrkB tyrosine kinase, Tyrosine kinase receptor B - 基因ID
- 4915
- 途径
- RTK signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport
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