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- 抗原 See all MC3R 抗体
- MC3R (Melanocortin 3 Receptor (MC3R))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MC3R antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human MC3 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MC5R (78 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human (100%) Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Pig (94%) Bat, Rabbit, Platypus (89%) Opossum, Turkey, Chicken, Xenopus, Trout (83%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from C-Terminus of human MC3 Receptor. Percent identity with other species by BLAST analysis: Human (100%), Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Pig (94%), Bat, Rabbit, Platypus (89%), Opossum, Turkey, Chicken, Xenopus, Trout (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product MC3R Primary Antibody
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anti-Melanocortin 3 Receptor (MC3R) (AA 131-230) antibody
MC3R 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 3 Receptor (MC3R) (AA 38-74) antibodyMC3R 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 3 Receptor (MC3R) (N-Term) antibodyVerified MC3R 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Melanocortin 3 Receptor (MC3R) (1st Extracellular Loop), (AA 102-117) antibodyMC3R 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 3 Receptor (MC3R) (AA 301-335), (C-Term) antibodyMC3R 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB48193 unconjugated
anti-Melanocortin 3 Receptor (MC3R) (N-Term) antibodyMC3R 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 3 Receptor (MC3R) (AA 1-74) antibodyMC3R 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Melanocortin 3 Receptor (MC3R) (C-Term) antibodyMC3R 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 3 Receptor (MC3R) (AA 1-120) antibodyMC3R 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 3 Receptor (MC3R) (AA 86-112) antibodyMC3R 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB22917 unconjugated
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- 应用备注
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Approved: IHC, IHC-P (10 - 12 μg/mL)
Usage: Immunohistochemistry with formalin-fixed paraffin-embedded tissues requires pretreatment with Proteinase K. - 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- MC3R (Melanocortin 3 Receptor (MC3R))
- 别名
- MC3R / MC3 Receptor (MC3R 产品)
- 别名
- zgc:194235 antibody, MC3-R antibody, BMIQ9 antibody, MC3 antibody, OB20 antibody, OQTL antibody, melanocortin 3 receptor antibody, mc3r antibody, MC3R antibody, Mc3r antibody
- 背景
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Name/Gene ID: MC3R
Subfamily: Melanocortin
Family: GPCR
Synonyms: MC3R, BMIQ9, MC3 receptor, Melanocortin receptor 3, OB20, MC3, MC3-R, Melanocortin 3 receptor, OQTL - 基因ID
- 4159
- 途径
- cAMP Metabolic Process
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