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- 抗原 See all LHCGR 抗体
- LHCGR (Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LHCGR antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human hCG receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey, Marmoset, Rat, Dog, Panda (95%) Bovine, Bat, Rabbit, Horse (89%) Ferret (84%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 19 amino acid peptide from C-terminus of human hCG receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Marmoset, Rat, Dog, Panda (95%), Bovine, Bat, Rabbit, Horse (89%), Ferret (84%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product LHCGR Primary Antibody
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anti-Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) (C-Term) antibody
LHCGR 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) antibodyLHCGR 适用: 人 WB, FACS, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 8G9A2 unconjugated
anti-Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) (AA 27-363) antibodyLHCGR 适用: 人 WB, IF, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) (AA 201-300) antibodyLHCGR 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) (C-Term) antibodyVerified LHCGR 适用: 人 IF, FACS, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) (AA 622-699) antibodyLHCGR 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) (AA 200-360) antibodyLHCGR 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) (AA 127-143), (N-Term) antibodyLHCGR 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) (C-Term) antibodyLHCGR 适用: 人 WB, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) (AA 27-362) antibodyLHCGR 适用: 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- LHCGR (Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR))
- 别名
- LHCGR / LHR / LH Receptor (LHCGR 产品)
- 别名
- HHG antibody, LCGR antibody, LGR2 antibody, LH/CG-R antibody, LH/CGR antibody, LHR antibody, LHRHR antibody, LSH-R antibody, ULG5 antibody, Gpcr19-rs1 antibody, LH-R antibody, Lhr antibody, LSHR antibody, lhr antibody, GTHR-II antibody, zfGTHR-II antibody, LHCGR antibody, LOC100222883 antibody, lh-r antibody, LOC100305044 antibody, luteinizing hormone/choriogonadotropin receptor antibody, luteinizing hormone/choriogonadotropin receptor L homeolog antibody, luteinizing hormone receptor antibody, LHCGR antibody, Lhcgr antibody, lhcgr antibody, lhcgr.L antibody, lhr antibody, LOC100305044 antibody
- 背景
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Name/Gene ID: LHCGR
Subfamily: Glycoprotein hormone
Family: GPCR
Synonyms: LHCGR, HCG receptor, HHG, LCGR, LH/CGR, LHR, LH receptor, LHRHR, Lutropin receptor, LGR2, LH/CG-R, LSH-R, ULG5, Luteinizing hormone receptor - 基因ID
- 3973
- 途径
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, cAMP Metabolic Process, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
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